Preparation of Functional Human Hepatocytes Ex Vivo.
Preparation of Functional Human Hepatocytes Ex Vivo.
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离体功能性人肝细胞的制备。
DOI：
10.1007/978-1-0716-2557-6_20
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发表时间：
2022
影响因子：
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通讯作者：
Okumura A and Tanimizu N
中科院分区：
文献类型：
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作者：
Hori Kosuke;Higashida Shinichi;Osaki Tomohiro;Kawano Tsuyoshi;Inaba Hiroshi;Matsuura Kazunori;Iwasaki Takashi;Okumura A and Tanimizu N
肝细胞是参与执行各种代谢反应的肝实质细胞。在治疗药物的开发过程中，在临床试验前使用肝细胞培养物进行毒理学测定。然而，由于原代肝细胞不能在体外增殖并迅速丧失其功能，因此人们付出了许多努力来改变培养条件以扩增原代肝细胞并诱导内在和外在干/祖细胞中的肝细胞功能。在本章中，我们总结了制备肝细胞培养物和从各种细胞来源诱导肝细胞的最新进展。
Hepatocytes are liver parenchymal cells involved in performing various metabolic reactions. During the development of therapeutic drugs, toxicological assays are conducted using hepatocyte cultures before clinical trials. However, since primary hepatocytes cannot proliferate and rapidly lose their functions in vitro, many efforts have been put into modifying culture conditions to expand primary hepatocytes and induce hepatocyte functions in intrinsic and extrinsic stem/progenitor cells. In this chapter, we summarize recent advances in preparing hepatocyte cultures and induction of hepatocytes from various cellular sources.