Star-shaped polymers for DNA sequencing by capillary electrophoresis.
Star-shaped polymers for DNA sequencing by capillary electrophoresis.
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DOI：
10.1016/j.chroma.2011.03.027
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发表时间：
2011-05
期刊：
影响因子：
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通讯作者：
Fan Gao;Cai Tie;Xinxiang Zhang;Zhiqiang Niu;Xiaojin He;Yuguo Ma
中科院分区：
文献类型：
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作者：
Fan Gao;Cai Tie;Xinxiang Zhang;Zhiqiang Niu;Xiaojin He;Yuguo Ma
DNA的分离和测序是人类基因组计划的主要目标，该计划也对基因分析和疾病诊断非常有用。毛细管电泳（CE）是DNA分离和分析的最常用技术之一。DNA分离通常使用毛细管凝胶电泳（CGE）模式来实现，其中聚合物凝胶被填充到毛细管中。与传统的CGE基质相比，亲水性聚合物基质可以被毛细管壁吸附，具有许多优点，包括稳定性，重现性和易于自动化。各种水溶性添加剂，如线性聚丙烯酰胺（PAA）和聚（N，N-二甲基丙烯酰胺）（PDMA），已被用作介质。在本研究中，设计并合成了不同的星形PDMA聚合物，以实现较低的聚合物溶液粘度。使用这些聚合物的DNA分离避免了高粘度和长分离时间的缺点，同时保持高分辨率（271 bp和281 bp之间的10 bp）。研究了聚合物浓度和结构对DNA分离的影响，发现聚合物浓度越高，分离效果越好，星形聚合物的分离效果比线型聚合物的好上级。这项工作表明，聚合物结构的修改是一个潜在的策略，优化DNA分离。
The separation and sequencing of DNA are the main objectives of the Human Genome Project, and this project has also been very useful for gene analysis and disease diagnosis. Capillary electrophoresis (CE) is one of the most common techniques for the separation and analysis of DNA. DNA separations are usually achieved using capillary gel electrophoresis (CGE) mode, in which polymer gel is packed into the capillary. Compared with a traditional CGE matrix, a hydrophilic polymer matrix, which can be adsorb by the capillary wall has numerous advantages, including stability, reproducibility and ease of automation. Various water-soluble additives, such as linear poly(acrylamide) (PAA) and poly(N,N-dimethylacrylamide) (PDMA), have been employed as media. In this study, different star-shaped PDMA polymers were designed and synthesized to achieve lower polymer solution viscosity. DNA separations with these polymers avoid the disadvantages of high viscosity and long separation time while maintaining high resolution (10 bp between 271 bp and 281 bp). The influences of the polymer concentration and structure on DNA separation were also determined in this study; higher polymer concentration yielded better separation performance, and star-like polymers were superior to linear polymers. This work indicates that modification of the polymer structure is a potential strategy for optimizing DNA separation.