Buccal Fat Pad, an Oral Access Source of Human Adipose Stem Cells with Potential for Osteochondral Tissue Engineering: An In Vitro Study
Buccal Fat Pad, an Oral Access Source of Human Adipose Stem Cells with Potential for Osteochondral Tissue Engineering: An In Vitro Study
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DOI：
10.1089/ten.tec.2009.0487
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发表时间：
2010-10-01
影响因子：
3
通讯作者：
Casals, Nuria
中科院分区：
文献类型：
--
作者：
Farre-Guasch, Elisabet;Marti-Pages, Carles;Casals, Nuria
干细胞为组织工程提供了一种有趣的工具，但临床应用受到供体部位发病率和收获时细胞数量少的限制。最近的研究已经确定了皮下脂肪组织中干细胞的丰富来源。脂肪干细胞（Adipose stem cells, ASCs）存在于脂肪组织中，能够分化为多个谱系，表达多种生长因子，适合临床应用。口腔脂肪垫（BFP）是一种在口腔内发现的脂肪包裹的肿块，对于牙医和口腔外科医生来说是一个容易获得的来源。分析从新鲜脂肪组织中获得的间质血管部分和传代的ASCs，以检测和量化该组织中ASCs的百分比。在这里，我们发现BFP包含一群干细胞，它们与来自腹部皮下脂肪组织的ASCs具有相似的表型，并且也能够分化成软骨、脂肪和成骨谱系。这些结果将BFP定义为一种新的、丰富的、可获得的用于组织工程目的的ASCs来源。
Stem cells offer an interesting tool for tissue engineering, but the clinical applications are limited by donor-site morbidity and low cell number upon harvest. Recent studies have identified an abundant source of stem cells in subcutaneous adipose tissue. Adipose stem cells (ASCs) present in adipose tissue are able to differentiate to several lineages and express multiple growth factors, which makes them suitable for clinical application. Buccal fat pad (BFP), an adipose-encapsulated mass found in the oral cavity, could represent an easy access source for dentists and oral surgeons. The stromal vascular fraction obtained from fresh BFP-derived adipose tissue and passaged ASCs were analyzed to detect and quantify the percentage of ASCs in this tissue. Here we show that BFP contains a population of stem cells that share a similar phenotype with ASCs from abdominal subcutaneous fat tissue, and are also able to differentiate into the chondrogenic, adipogenic, and osteogenic lineage. These results define BFP as a new, rich, and accessible source of ASCs for tissue engineering purposes.