High Dynamic Range Fluorescence Imaging
High Dynamic Range Fluorescence Imaging
复制标题
DOI：
10.1109/jstqe.2018.2881608
复制
发表时间：
2019-01
影响因子：
4.9
通讯作者：
Claudio Vinegoni;P. Fumene Feruglio;R. Weissleder
中科院分区：
文献类型：
--
作者：
Claudio Vinegoni;P. Fumene Feruglio;R. Weissleder
在高动态范围上的荧光采集和图像显示是非常期望的。然而，当前光电检测器和成像电荷耦合器件的有限动态范围对可以在单个图像采集期间同时捕获的荧光强度施加了限制。当对生物样品进行成像时，这是特别麻烦的，其中蛋白质表达波动很大。因此，生物图像通常包含信号饱和或隐藏在背景噪声中的区域，导致信息丢失。在本文中，我们总结了最近的工作，从我们的小组和其他人，扩展到传统的高动态范围荧光成像。这些策略具有许多生物学应用，例如神经连接的映射、血管成像、生物分布研究或单细胞和器官水平的药理学成像。
Fluorescence acquisition and image display over a high dynamic range is highly desirable. However, the limited dynamic range of current photodetectors and imaging charge-coupled devices impose a limit on the fluorescence intensities that can be simultaneously captured during a single image acquisition. This is particularly troublesome when imaging biological samples, where protein expression fluctuates considerably. As a result, biological images will often contain regions with signal that is either saturated or hidden within background noise, causing information loss. In this paper, we summarize recent work from our group and others, to extended conventional to high dynamic range fluorescence imaging. These strategies have many biological applications, such as mapping of neural connections, vascular imaging, bio-distribution studies or pharmacologic imaging at the single cell and organ level.