Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment.
Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment.
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DOI：
10.1016/j.biortech.2007.12.027
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发表时间：
2008-09
影响因子：
11.4
通讯作者：
F. Sun;Hongzhang Chen
中科院分区：
文献类型：
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作者：
F. Sun;Hongzhang Chen
考虑到油脂工业中甘油加工的实际技术经济性，提出了进一步探索常压水相甘油自催化有机溶剂预处理（AAGAOP）提高木质纤维素生物质酶解效果的研究方向。在液固比为20 gg-1、温度为220°C、时间为3 h的条件下，AAGAOP可使麦草半纤维素和木质素的去除率分别达到70%和65%以上，纤维素的保留率达到98%以上。预处理后的纤维在48 h后酶解得率可达90%以上。烘干过程中，脱水容易引起纤维的角化，导致干纤维的酶水解率较低。通过扫描电镜观察，AAGAOP将小麦秸秆破碎成细而细的纤维，具有较小的平均尺寸和较大的表面积。AAGAOP技术作为一种新的策略，通过消除化学组成障碍和改变物理结构障碍来增强木质纤维素生物质的酶解。
Considering the practical technology–economy of glycerol processing from oleochemicals industry, the ensuing work was proposed to further explore the atmospheric aqueous glycerol autocatalytic organosolv pretreatment (AAGAOP) to improve the enzymatic hydrolysis of lignocellulosic biomass. With the liquid–solid ratio of 20gg−1at 220°C for 3h, the AAGAOP enabled wheat straw to remove ∼70% hemicelluloses and ∼65% lignin, with ∼98% cellulose retention. The pretreated fiber was achieved with ∼90% of the enzymatic hydrolysis yield after 48h. At oven-drying, dehydration was likely to cause the hornification of fiber, which was responsible for the low enzymatic hydrolysis of dried fiber. With SEM observations, the AAGAOP disrupted wheat straw into thin and fine fibrils, with a small average size and more surface area. The AAGAOP technique, as a novel strategy, enhanced the enzymatic hydrolysis of lignocellulosic biomass by removing the chemically compositional barrier and altering the physically structural impediment.