Parallelized STED fluorescence nanoscopy
Parallelized STED fluorescence nanoscopy
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DOI：
10.1364/oe.19.023716
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发表时间：
2011-11-21
期刊：
影响因子：
3.8
通讯作者：
Hell, Stefan W.
中科院分区：
文献类型：
--
作者：
Bingen, Pit;Reuss, Matthias;Hell, Stefan W.
我们引入了一种并行 STED 显微镜，具有 m = 4 对扫描激发和 STED 光束，可将给定样品区域的成像速度提高 m 倍，同时基本上保持单光束扫描的所有优点。该装置仅需要单个激光源和光纤输入，本质上在空间和时间上都是对齐的。如果有足够的激光功率，该设计可以轻松扩展到更高程度的并行化。 (C) 2011 美国光学学会
We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m. (C) 2011 Optical Society of America