IN-SITU HYBRIDIZATION SHOWS DISTINCT PATTERNS OF MUCIN GENE-EXPRESSION IN NORMAL, BENIGN, AND MALIGNANT PANCREAS TISSUES
IN-SITU HYBRIDIZATION SHOWS DISTINCT PATTERNS OF MUCIN GENE-EXPRESSION IN NORMAL, BENIGN, AND MALIGNANT PANCREAS TISSUES
复制标题
DOI：
10.1016/0016-5085(95)90406-9
复制
发表时间：
1995-09-01
期刊：
影响因子：
29.4
通讯作者：
REAL, FX
中科院分区：
文献类型：
--
作者：
BALAGUE, C;AUDIE, JP;REAL, FX
背景与目的：Northern印迹和免疫组织化学已经显示了胰腺粘蛋白基因表达的细胞特异性模式以及与肿瘤转化相关的改变。本研究的目的是确定正常胰腺和胰腺良性和恶性增生性病变组织样本中单细胞水平粘蛋白转录本的存在。方法：石蜡包埋组织切片，用S-35标记寡核苷酸进行原位杂交。结果：腺泡细胞仅表达MUC1。正常胰管表现为MUC1和MUC5B的同源表达和MUC3的异质性表达。MUC2、MUC4和MUC5AC通常在正常胰腺组织的所有细胞中都检测不到。胰腺癌通常表达MUC1、MUC3、MUC4、MUC5B和MUC5AC。胰腺癌旁的慢性梗阻性胰腺炎表现出与正常导管相同的粘蛋白基因表达模式。在乳头状增生区，观察到MUC3、MUC5B和MUC5AC的表达改变。结论：原位杂交证实胰腺外分泌的肿瘤性转化伴随着粘蛋白基因表达的改变。虽然这种类型的改变并不局限于癌细胞，但本研究的发现表明，粘蛋白基因表达的分析可能对胰腺病变的鉴别诊断有价值。
Background & Aims: Northern blotting and immunohistochemistry have shown cell-specific patterns of mucin gene expression in the pancreas and alterations associated with neoplastic transformation. The aim of this study was to determine the presence of mucin transcripts at the single cell level in tissue samples from normal pancreas and benign and malignant pancreatic proliferative lesions. Methods: In situ hybridization with S-35-labeled oligonucleotides was performed on sections of paraffin-embedded tissues. Results: Acinar cells express only MUC1. Normal pancreatic ducts show homogeneous expression of MUC1 and MUC5B and heterogenous expression of MUC3. MUC2, MUC4, and MUC5AC are generally undetectable in all cells of normal pancreas tissues. Pancreas cancers generally express MUC1, MUC3, MUC4, MUC5B, and MUC5AC. Obstructive chronic pancreatitis adjacent to pancreas cancers shows the same pattern of mucin gene expression as normal ducts. In areas of papillary hyperplasia, altered expression of MUC3, MUC5B, and MUC5AC is observed. Conclusions: In situ hybridization has confirmed that neoplastic transformation of the exocrine pancreas is accompanied by changes in mucin gene expression. Although this type of change is not restricted to cancer cells, the findings of this study suggest that analysis of mucin gene expression may be of value in the differential diagnosis of pancreatic lesions.