A combination of Lactobacillus buchneri and Pediococcus pentosaceus extended the aerobic stability of conventional and brown midrib mutants–corn hybrids ensiled at low dry matter concentrations by causing a major shift in their bacterial and fungal community
A combination of Lactobacillus buchneri and Pediococcus pentosaceus extended the aerobic stability of conventional and brown midrib mutants–corn hybrids ensiled at low dry matter concentrations by causing a major shift in their bacterial and fungal community
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2021
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通讯作者：
Joo;Yuchen Zhao;M. Killerby;F. Tiezzi;E. Gutiérrez-Rodríguez
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作者：
Joo;Yuchen Zhao;M. Killerby;F. Tiezzi;E. Gutiérrez-Rodríguez
对4个高水分条件下收获的玉米杂种施用组合接种剂对其营养价值和微生物种群的影响进行了评价。处理设计为加（INO）和不加（CON）接种剂青贮玉米杂种的因子组合。杂种TMF2R737 （MCN）、F2F817 （MBR）、P2089YHR （PCN）和PI144XR （PBR）在干物质（DM）浓度分别为30.5%、26.3%、31.1%和31.5%的条件下青贮；MBR和PBR为棕色中脉突变体（BMR）。接种剂中含有布氏乳杆菌和戊糖小球菌（鲜玉米4 × 105和1 × 105 cfu/g）。该实验采用完全随机设计，重复处理6次。玉米分别用接种剂和不接种剂处理，装在7.6 L桶筒仓中，保存100 d。第0 d时，PBR中Enterobacteriaceae的相对丰度（RA, %）低于其他杂交种[51.3比¯x =（平均）58.4]，真菌中incertae setis (i.s。在常规杂交中，Tremellales和Mucoraceae的丰度分别高于或低于BMRs（¯x = 25.8 vs.¯x = 13.9，¯x = 3.64 vs.¯x = 7.52; P < 0.04）。青贮后，INO具有较高的乳酸（鲜玉米9.3比7.1 log cfu/g）和乙酸（DM 3.44%比1.32%），较低的酵母（3.1比4.6）和霉菌（1.5比3.0），并延长了有氧稳定性（582比111 h），但降低了DM回收率（95.6%比97.4%）（P < 0.02）。
We evaluated the effects of applying a combination inoculant to four corn hybrids harvested at high moisture on their nutritive value and microbial populations. The treatment design was the factorial combination of corn hybrids ensiled with (INO) and without (CON) inoculant. The hybrids were TMF2R737 (MCN), F2F817 (MBR), P2089YHR (PCN), and PI144XR (PBR), ensiled at dry matter (DM) concentrations of 30.5%, 26.3%, 31.1%, and 31.5%, respectively; MBR and PBR were brown midrib mutants (BMR). The inoculant contained Lactobacillus buchneri and Pediococcus pentosaceus (4 × 10 5 and 1 × 10 5 cfu/g of fresh corn). The experiment had a complete randomized design with treatments replicated six times. Corn was treated or not with inoculant, packed into 7.6 L bucket silos, and stored for 100 d. At d 0, the relative abundance (RA, %) of Enterobacteriaceae was lower in PBR vs. the other hybrids [51.3 vs. ¯ x = (average of) 58.4] and in the case of fungi, incertae sedis (i.s.) Tremellales and Mucoraceae were more and less abundant, respectively, in conventional hybrids vs. BMRs ( ¯ x = 25.8 vs. ¯ x = 13.9 and ¯ x = 3.64 vs. ¯ x = 7.52; P < 0.04). After ensiling, INO had higher LAB (9.3 vs. 7.1 log cfu/g of fresh corn) and acetic acid (3.44% vs. 1.32% of DM) and lower yeast (3.1 vs. 4.6) and molds (1.5 vs. 3.0), and also extended the aerobic stability (582 vs. 111 h) but decreased DM recovery (95.6% vs. 97.4%) vs. CON ( P < 0.02).