CARCINOGENICITY OF DIETHYLNITROSAMINE IN NEWBORN, INFANT, AND ADULT MICE
CARCINOGENICITY OF DIETHYLNITROSAMINE IN NEWBORN, INFANT, AND ADULT MICE
复制标题
DOI：
10.1007/bf00390974
复制
发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
RAO, KVN
中科院分区：
文献类型：
--
作者：
VESSELINOVITCH, SD;KOKA, M;RAO, KVN
用C57 BL/6 Jx、C3 HeB/FeJxA/JF 1（B6 C3 F1）和C3 HeB/FeJxA/JF 1（C3 AF 1）杂交小鼠研究了年龄、性别和品系对二乙基亚硝胺（DEN）致癌性的修饰作用。每只动物接受4次1.5或3.0 μ g DEN/g体重的腹膜内注射。第一次注射在出生后第1、15或42天进行。随后的治疗分别以3天、6天和6天的间隔进行。观察小鼠的剩余寿命。DEN处理诱发的肿瘤在肝、肺和前胃中的频率从高到低。诱导的肝肿瘤大多数是肝细胞癌。作为新生儿和婴儿治疗的动物比作为年轻成年人治疗的动物发生更多的肝脏肿瘤。新生儿和婴儿雌性动物在较晚年龄（B6 C3 F1）发生肝脏肿瘤，并且发生率（C3 AF 1）低于接受类似治疗的雄性动物。B6 C3 F1小鼠比C3 AF 1小鼠发生更多的肝细胞癌和更高的肺转移率。相比之下，C3 AF 1小鼠比B6 C3 F1混合体具有更高的发病率和多样性。在C3 AF 1小鼠中也观察到前胃肿瘤，发生率略高但显著较高。
Modifying effects of age, sex and mouse strain on diethylnitrosamine (DEN) carcinogenesis were investigated in C57BL/6Jx C3HeB/FeJ F1 (B6C3F1) and C3HeB/FeJxA/J F1 (C3AF1) hybrid mice. Animals each received 4 i.p. injections of 1.5 or 3.0 .mu.g DEN/g body wt. The first injections were administered on days 1, 15 or 42 of life. Subsequent treatments were delivered at 3-, 6- and 6-day intervals, respectively. Mice were kept under observation for the remaining life-span. DEN treatment induced tumors in liver, lungs and forestomach in descending order of frequency. The majority of the induced liver tumors were hepatocellular carcinomas. Animals treated as newborns and infants developed significantly more liver tumors than animals that were treated as young adults. Newborn and infant females developed liver tumors at a later age (B6C3F1) and with a lower incidence (C3AF1) than similarly treated males. The B6C3F1 mice developed more hepatocellular carcinomas and a higher rate of pulmonary metastases than the C3AF1 mice. In contrast, C3AF1 mice developed lung tumors with a higher incidence and multiplicity than B6C3F1 hydbrids. Forestomach tumors were observed also with a slightly but significantly higher incidence in C3AF1 mice.