Azole-resistant Aspergillus fumigatus in Denmark: a laboratory-based study on resistance mechanisms and genotypes
Azole-resistant Aspergillus fumigatus in Denmark: a laboratory-based study on resistance mechanisms and genotypes
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DOI：
10.1016/j.cmi.2016.04.001
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发表时间：
2016-06-01
影响因子：
14.2
通讯作者：
Arendrup, M. C.
中科院分区：
文献类型：
--
作者：
Jensen, R. H.;Hagen, F.;Arendrup, M. C.
在丹麦的临床环境中，源于环境并在治疗过程中诱导产生的耐氮唑烟曲霉菌不断出现。我们在丹麦国家真菌学参考实验室进行了一项以实验室为基础的回顾研究(2010-2014)，研究了烟曲霉菌的唑类耐药性和遗传关系。通过形态学、耐热性和/或β-微管蛋白测序，共鉴定了1162株临床和133株环境中的烟曲霉菌。用唑类琼脂进行唑类耐药筛选，药敏试验采用欧洲药敏试验委员会E.Def 9.2参考方法和细胞色素P51a测序。对暴发疫情进行了基因分型，并在适当的情况下进行了短串联重复序列烟曲霉微卫星分析。133株环境分离的烟曲霉菌均对唑类敏感。然而，从2010年到2014年，对唑类药物的耐药率有所上升(从1.4%上升到6%(p
Azole-resistant Aspergillus fumigatus originating from the environment as well as induced during therapy are continuously emerging in Danish clinical settings. We performed a laboratory-based retrospective study (2010-2014) of azole resistance and genetic relationship of A. fumigatus at the national mycology reference laboratory of Denmark. A total of 1162 clinical and 133 environmental A. fumigatus isolates were identified by morphology, thermotolerance and/or beta-tubulin sequencing. Screening for azole resistance was carried out using azole agar, and resistant isolates were susceptibility tested by the EUCAST (European Committee on Antimicrobial Susceptibility Testing) E. Def 9.2 reference method and CYP51A sequenced. Genotyping was performed for outbreak investigation and, when appropriate, short tandem repeat Aspergillus fumigatus microsatellite assay. All 133 environmental A. fumigatus isolates were azole susceptible. However, from 2010 to 2014, there was an increasing prevalence of azole resistance (from 1.4 to 6% isolates (p