In vitro morphogenesis of chick embryo hypertrophic cartilage.
In vitro morphogenesis of chick embryo hypertrophic cartilage.
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DOI：
10.1083/jcb.105.2.999
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发表时间：
1987-08
期刊：
影响因子：
--
通讯作者：
Cancedda R
中科院分区：
文献类型：
--
作者：
Tacchetti C;Quarto R;Nitsch L;Hartmann DJ;Cancedda R
去分化鸡胚软骨细胞(Castagnola， P.， G. Moro， F. Descalzi-Cancedda， R. Cancedda, 1986，细胞生物学杂志。， 102:2310- 2317)，当转移到琼脂糖包被的培养皿中，在抗坏血酸的存在下，聚集并保持簇状。随着培养时间的推移，簇的大小会逐渐增大，并相互粘附，形成尺寸可能为几毫米的结构。培养7天后，这些结构具有成熟的肥大软骨的组织学外观，部分被一层类似软骨膜的细长细胞包围。聚集体内的细胞根据其位置的不同，具有I期（增殖）或II期（肥大）软骨细胞的超微结构特征。I型、II型和X型胶原在体外形成的软骨中不同培养时间的出现和分布，显示出这些抗原在时间和空间上的分布，使人联想到体内软骨中发生的成熟事件。从单个克隆的去分化软骨细胞中获得的细胞群也显示出类似的组织学外观。
Dedifferentiated chick embryo chondrocytes (Castagnola, P., G. Moro, F. Descalzi-Cancedda, and R. Cancedda, 1986, J. Cell Biol., 102:2310- 2317), when transferred to suspension culture on agarose-coated dishes in the presence of ascorbic acid, aggregate and remain clustered. With time in culture, clusters grow in size and adhere to each other, forming structures that may be several millimeters in dimension. These structures after 7 d of culture have the histologic appearance of mature hypertrophic cartilage partially surrounded by a layer of elongated cells resembling the perichondrium. Cells inside the aggregates have ultrastructural features of stage I (proliferating) or stage II (hypertrophic) chondrocytes depending on their location. Occurrence and distribution of type I, II, and X collagens in the in vitro-formed cartilage at different times of culture, show a temporal and spatial distribution of these antigens reminiscent of the maturation events occurring in the cartilage in vivo. A comparable histologic appearance is shown also by cell aggregates obtained starting with a population of cells derived from a single, cloned, dedifferentiated chondrocyte.