Identification of carboxypeptidase E and γ-glutamyl hydrolase as biomarkers for pulmonary neuroendocrine tumors by cDNA microarray
Identification of carboxypeptidase E and γ-glutamyl hydrolase as biomarkers for pulmonary neuroendocrine tumors by cDNA microarray
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DOI：
10.1016/j.humpath.2004.06.014
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发表时间：
2004-10-01
期刊：
影响因子：
3.3
通讯作者：
Harris, CC
中科院分区：
文献类型：
--
作者：
He, P;Varticovski, L;Harris, CC
肺神经内分泌肿瘤的恶性行为差异很大。根据组织学检查对它们进行分类往往很困难。为了寻找这些肿瘤的分子和预后标志物，我们使用了针对具有良好特征的永生化的支气管上皮细胞系BEAS-2B的参考RNA的人转录本的cDNA微阵列分析。用激光捕获显微切割法从17例典型类癌、小细胞肺癌和大细胞神经内分泌癌的原发肿瘤中分离出肿瘤细胞。根据所提出的组织学分类，无监督的分层聚类算法导致了对每种肿瘤亚型的精确分类。使用监督分析对基因进行选择，最终确定了198个有统计学意义的基因(P
Pulmonary neuroendocrine tumors vary dramatically in their malignant behavior. Their classification, based on histological examination, is often difficult. In search of molecular and prognostic markers for these tumors, we used cDNA microarray analysis of human transcripts against reference RNA from a well-characterized immortalized bronchial epithelial cell line, BEAS-2B. Tumor cells were isolated by laser-capture microdissection from primary tumors of 17 typical carcinoids, small cell lung cancers, and large cell neuroendocrine carcinomas. An unsupervised, hierarchical clustering algorithm resulted in a precise classification of each tumor subtype according to the proposed histological classification. Selection of genes, using supervised analysis, resulted in the identification of 198 statistically significant genes (P