Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea.
Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea.
复制标题
用于标记耳蜗中 I 型和 II 型传入神经元的转基因小鼠系的表征。
DOI：
10.1038/s41598-019-41770-5
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发表时间：
2019
影响因子：
4.6
通讯作者：
Fuchs,PaulAlbert
中科院分区：
文献类型：
--
作者：
Vyas,Pankhuri;Wu,JingjingSherry;Jimenez,Adrian;Glowatzki,Elisabeth;Fuchs,PaulAlbert
耳蜗由I型和II型传入神经元支配。I型传入神经是有髓鞘的、直径较大的神经元，其发送单个树突以接触单个内毛细胞，而无髓鞘的II型传入神经数量较少，并且接收来自许多外毛细胞的输入。这种显著分化的神经支配模式强烈表明了专门的功能。这些功能可以用特定的遗传标记来研究，这些标记可以标记和操纵每个传入类，而不会显著影响其他类。在此，我们对三种小鼠模型进行了I型和II型耳蜗传入神经元特异性标记的表征和测试：Nos 1CreER小鼠表现出对I型传入神经元的选择性标记，Slc 6a 4-GFP小鼠对II型传入神经元的标记具有轻微的耳蜗顶侧偏好，而Drd 2-Cremice则选择性标记耳蜗基底附近的II型传入神经元。结合Th 2A-CreER和CGRP α-EGFP标记II型纤维，本文报道的小鼠系构成了一个有前途的工具包，用于I型和II型耳蜗传入纤维的遗传操作。
The cochlea is innervated by type I and type II afferent neurons. Type I afferents are myelinated, larger diameter neurons that send a single dendrite to contact a single inner hair cell, whereas unmyelinated type II afferents are fewer in number and receive input from many outer hair cells. This strikingly differentiated innervation pattern strongly suggests specialized functions. Those functions could be investigated with specific genetic markers that enable labeling and manipulating each afferent class without significantly affecting the other. Here three mouse models were characterized and tested for specific labeling of either type I or type II cochlear afferents.Nos1CreERmice showed selective labeling of type I afferent fibers,Slc6a4-GFPmice labeled type II fibers with a slight preference for the apical cochlea, andDrd2-Cremice selectively labeled type II afferent neurons nearer the cochlear base. In conjunction with theTh2A-CreERandCGRPα-EGFPlines described previously for labeling type II fibers, the mouse lines reported here comprise a promising toolkit for genetic manipulations of type I and type II cochlear afferent fibers.