Genetic Manipulation of Biosynthetic Pathways in Mint.
Genetic Manipulation of Biosynthetic Pathways in Mint.
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DOI：
10.3389/fpls.2022.928178
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发表时间：
2022
影响因子：
5.6
通讯作者：
Scofield, Simon
中科院分区：
文献类型：
--
作者：
Fuchs, Lorenz K.;Holland, Alistair H.;Ludlow, Richard A.;Coates, Ryan J.;Armstrong, Harvey;Pickett, John A.;Harwood, John L.;Scofield, Simon
近年来，对芳香植物的研究有所增加，工业，学术和制药行业都有很大的兴趣。在植物中吸引越来越多的注意力是薄荷属。（薄荷），唇形科（Lamiaceae）的成员。薄荷精油包含被称为萜类化合物/类异戊二烯的多种分子，有机化学品是天然植物衍生化合物中最多样化的一类。几种薄荷属植物的萜类化合物谱。薄荷醇是一种环状单萜，具有一些显著的生物学特性，使其可用于制药、医疗、化妆品和清洁产品工业。随着薄荷精油全球市场的增长，人们希望改善精油的组成和产量。单萜类化合物生物合成途径被很好地表征，因此已经进行了代谢工程尝试以促进这种改善。本文对薄荷属植物的研究进展进行了综述。并试图改变通过生物合成途径的碳通量以增加产率和提高精油的组成。这包括通过过表达和RNAi抑制来操纵内源和异源生物合成酶。基因参与的MEP途径，薄荷醇和香芹酮的生物合成途径和转录因子已知影响次生代谢将讨论沿着非代谢工程的方法，包括环境因素和使用植物生长调节剂。
In recent years, the study of aromatic plants has seen an increase, with great interest from industrial, academic, and pharmaceutical industries. Among plants attracting increased attention are the Mentha spp. (mint), members of the Lamiaceae family. Mint essential oils comprise a diverse class of molecules known as terpenoids/isoprenoids, organic chemicals that are among the most diverse class of naturally plant derived compounds. The terpenoid profile of several Mentha spp. is dominated by menthol, a cyclic monoterpene with some remarkable biological properties that make it useful in the pharmaceutical, medical, cosmetic, and cleaning product industries. As the global market for Mentha essential oils increases, the desire to improve oil composition and yield follows. The monoterpenoid biosynthesis pathway is well characterised so metabolic engineering attempts have been made to facilitate this improvement. This review focuses on the Mentha spp. and attempts at altering the carbon flux through the biosynthetic pathways to increase the yield and enhance the composition of the essential oil. This includes manipulation of endogenous and heterologous biosynthetic enzymes through overexpression and RNAi suppression. Genes involved in the MEP pathway, the menthol and carvone biosynthetic pathways and transcription factors known to affect secondary metabolism will be discussed along with non-metabolic engineering approaches including environmental factors and the use of plant growth regulators.
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DOI：
10.1073/pnas.96.23.13062
发表时间：
1999-11-09
影响因子：
11.1
作者：
Burke, CC;Wildung, MR;Croteau, R
通讯作者：
Croteau, R
DOI：
10.1016/0006-3002(61)90612-6
发表时间：
1961-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BATTAILE, J;LOOMIS, WD
通讯作者：
LOOMIS, WD
影响因子：
3.9
作者：
Bertea, CM;Schalk, M;Croteau, R
通讯作者：
Croteau, R
影响因子：
3.9
作者：
Burke, C;Klettke, K;Croteau, R
通讯作者：
Croteau, R
影响因子：
4.8
作者：
Burke, C;Croteau, R
通讯作者：
Croteau, R