Serp-1 Promotes Corneal Wound Healing by Facilitating Re-epithelialization and Inhibiting Fibrosis and Angiogenesis.
Serp-1 Promotes Corneal Wound Healing by Facilitating Re-epithelialization and Inhibiting Fibrosis and Angiogenesis.
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DOI：
10.3389/fcvm.2021.649124
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发表时间：
2021
影响因子：
3.6
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Ju B;Guo O;Benissan-Messan DZ;Shawver MH;Chen P;Geng B;Wei S;Yaron JR;Lucas AR;Zhu H
目的：化学性角膜损伤的发病率很高，通常会导致视力障碍。在这里，我们研究了 Serp-1（一种丝氨酸蛋白酶抑制剂）在角膜伤口愈合中的作用。方法：对 14 只小鼠进行碱诱导角膜损伤。受伤后，5 只小鼠每天接受局部生理盐水涂抹，而 9 只小鼠接受局部 Serp-1 100 μL 联合每日皮下注射 100 ng/克体重的 Serp-1。每天通过荧光素染色和成像监测角膜损伤。获得角膜横截面H&E染色。 CD31被用作新血管形成的标志物。结果：Serp-1 通过减少纤维化和新生血管形成来促进角膜伤口愈合，同时减轻炎症细胞浸润，且没有与其应用相关的明显危害。结论：Serp-1 可有效减轻角膜损伤小鼠模型中的炎症、减少纤维化并减少新生血管形成，而不影响其他器官。转化相关性：我们的研究为局部应用 Serp-1 治疗角膜伤口提供了临床前数据。
Purpose: Chemical corneal injuries carry a high morbidity and commonly lead to visual impairment. Here, we investigate the role of Serp-1, a serine protease inhibitor, in corneal wound healing. Methods: An alkaline-induced corneal injury was induced in 14 mice. Following injury, five mice received daily topical saline application while nine mice received Serp-1 100 μL topically combined with a daily subcutaneous injection of 100 ng/gram body weight of Serp-1. Corneal damage was monitored daily through fluorescein staining and imaging. Cross sectional corneal H&E staining were obtained. CD31 was used as marker for neovascularization. Results: Serp-1 facilitates corneal wound healing by reducing fibrosis and neovascularization while mitigating inflammatory cell infiltration with no noticeable harm related to its application. Conclusions: Serp-1 effectively mitigates inflammation, decreases fibrosis, and reduce neovascularization in a murine model of corneal injury without affecting other organs. Translational Relavence: Our study provides preclinical data for topical application of Serp-1 to treat corneal wounds.