Cloning and characterization of the murine activin receptor like kinase-1 (ALK-1) homolog.
Cloning and characterization of the murine activin receptor like kinase-1 (ALK-1) homolog.
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DOI：
10.1006/bbrc.1995.2594
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发表时间：
1995-11
影响因子：
3.1
通讯作者：
Xiying Wu;C. E. Robinson;H. Fong;Judy S. Crabtree;B. R. Rodríguez;Bruce A. Roe;J. Gimble
中科院分区：
文献类型：
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作者：
Xiying Wu;C. E. Robinson;H. Fong;Judy S. Crabtree;B. R. Rodríguez;Bruce A. Roe;J. Gimble
在骨髓基质细胞系中检测到多种丝氨酸/苏氨酸激酶。其中之一，鼠激活素受体样激酶-1（ALK-1）同源物被克隆和测序。表达的重组蛋白（62 kDa）与cDNA预测的大小（58.6 kDa）一致。在Western印迹上，山羊多克隆抗体检测骨髓基质细胞、肺、脑、肾和脾中的天然ALK-1蛋白。检测到60 kDa和72-76 kDa的两种蛋白质种类。糖基化事件或选择性剪接可以解释较大的蛋白质种类。
Multiple serine/threonine kinases were detected in a bone marrow stromal cell line. One of these, the murine activin receptor like kinase-1 (ALK-1) homolog was cloned and sequenced. The expressed recombinant protein (62 kDa) was consistent in size with that predicted by the cDNA (58.6 kDa). On Western blots, a goat polyclonal antibody detected the native ALK-1 protein in bone marrow stromal cells, lung, brain, kidney and spleen. Two protein species of 60 kDa and 72-76 kDa were detected. Glycosylation events or alternative splicing may account for the larger protein specie.