Genetic diversity of Fasciola hepatica in Spain and Peru
Genetic diversity of Fasciola hepatica in Spain and Peru
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DOI：
10.1016/j.parint.2020.102100
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发表时间：
2020-06-01
影响因子：
1.9
通讯作者：
Ichikawa-Seki, Madoka
中科院分区：
文献类型：
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作者：
Tran Nhat Thang;Vazquez-Prieto, Severo;Ichikawa-Seki, Madoka
在本研究中，来自西班牙的片形吸虫的分子特征，以揭示与先前报道的秘鲁F。肝细胞应用核DNA标记磷酸烯醇丙酮酸羧激酶（pepck）和DNA聚合酶δ（pold）对片形吸虫进行种间鉴定。共检出片形吸虫196株，经鉴定为F. pepck和pold分析表明，线粒体NADH脱氢酶亚基1（nad1）共检测到26种单倍型。其中只有一个是以前在西班牙样品中发现的，这表明F.西班牙产的hepatica其中3个单倍型与秘鲁F.肝。成对注视指数值证实了西班牙和秘鲁F.肝组织标本西班牙样本的遗传变异性明显高于秘鲁人口。这些结果进行了讨论与假设的介绍寄生虫在美国从欧洲和最近的证据前西班牙F。来自阿根廷的肝脏化石
In the present study, molecular characterization of Fasciola flukes from Spain was performed to reveal the relation with the previously reported Peruvian F. hepatica population. The nuclear DNA markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), were used for species identification of Fasciola flukes. A total of 196 Fasciola flukes were identified as F. hepatica by pepck and pold, and 26 haplotypes were detected in mitochondrial NADH dehydrogenase subunit 1 (nad1). Only one of them was previously found in Spanish samples; which indicates the existence of high genetic diversity and population structure in F. hepatica from Spain. Three haplotypes were identical to those from Peruvian F. hepatica. The pairwise fixation index value confirmed a relatively close relationship between the Spanish and Peruvian F. hepatica samples. The Spanish samples showed clearly higher genetic variability than the Peruvian population. These results are discussed in relation with the hypothesis of the introduction of the parasite in America from Europe and recent evidence of pre-Hispanic F. hepatica from Argentina revealed by ancient DNA.