MiR-204/14-3-3ζ axis regulates osteosarcoma cell proliferation through SATA3 pathway.
MiR-204/14-3-3ζ axis regulates osteosarcoma cell proliferation through SATA3 pathway.
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DOI：
10.1692/ph.2017.7574
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发表时间：
2017-10
期刊：
影响因子：
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通讯作者：
R. Zhao;Hongbo He;Yong Zhu;Jun Wan;Yusheng Li;Shuguang Gao;Can Zhang
中科院分区：
文献类型：
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作者：
R. Zhao;Hongbo He;Yong Zhu;Jun Wan;Yusheng Li;Shuguang Gao;Can Zhang
细胞过度增殖是骨肉瘤（OS）的一个主要问题。因此，需要进一步阐明 OS 过度增殖的分子机制。 Western blots结果显示14-3-3ze蛋白在OS细胞系中表达上调； 14-3-3ze 敲低显着抑制 OS 细胞增殖以及 p-STAT3、c-Myc 和 Cyclin D1 的蛋白水平。 MicroRNA-204 (miR-204) 被认为是包括 OS 在内的癌症发生过程中的重要调节因子。在这里，我们发现miR-204直接靶向14-3-3ze的3'UTR来抑制其表达，从而抑制14-3-3ze诱导的OS细胞过度增殖。此外，我们证明 STAT3 通路参与 miR-204/14-3-3ze 对 OS 细胞增殖的调节。我们的研究结果提供了有关 miR-204/14-3-3ze 通过 STAT3 途径影响 OS 细胞增殖的潜在机制的信息，并表明 miR-204 和 14-3-3ze 作为 OS 的潜在治疗靶点。
Hyperproliferation of cells is a major problem is osteosarcoma (OS). So, further elucidation of the molecular mechanisms underlying hyperproliferation of OS is needed. Western blots results showed that 14-3-3ζ protein was upregulated in OS cell lines; 14-3-3ζ knockdown significantly suppressed OS cell proliferation, as well as the protein levels of p-STAT3, c-Myc and Cyclin D1. MicroRNA-204 (miR-204) has been regarded as an essential regulator in cancer carcinogenesis, including OS. Here, we revealed that miR-204 directly targets the 3'UTR of 14-3-3ζ to inhibit its expression, thus to suppress 14-3-3ζ -induced OS cell hyperproliferation. Further, we demonstrated that the STAT3 pathway was involved in miR-204/14-3-3ζ regulation of OS cell proliferation. Our findings provide information about the underlying mechanisms of miR-204/14-3-3ζ in OS cell proliferation through the STAT3 pathway, and suggest miR-204 and 14-3-3ζ as potential therapeutic targets in OS.