Obesity and Hypothalamic Inflammation from High Fat Diets of Different Fatty Acid Profile
Obesity and Hypothalamic Inflammation from High Fat Diets of Different Fatty Acid Profile
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DOI：
10.1096/fasebj.29.1_supplement.746.2
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
Kyle J. Mamounis;T. Roepke
中科院分区：
文献类型：
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作者：
Kyle J. Mamounis;T. Roepke
西方饮食的一个显著变化，在肥胖流行之前和持续同时，是脂肪酸谱从饱和和单不饱和脂肪酸向多不饱和脂肪酸的变化，主要是n-6亚油酸（LA）。本研究的目的是观察不同类型的脂肪酸在高脂饮食（HFD）中的肥胖诱导能力。将48只雄性C57 BL 6/J小鼠分为4个饮食处理组（n=12）：对照组（10% kcals脂肪）和3个HFD组（45% kcals脂肪），其中LA占45%脂肪的百分比不同。第一HFD具有来自亚油酸（LA）的1% kcal;第二HFD具有15% LA;并且第三HFD具有来自LA的22.5% kcal。LA的主要来源是大豆油，饱和脂肪的主要来源是椰子油。在12周研究期间，记录体重和摄食量。12周后，进行MRI身体成分，间接量热法和葡萄糖和胰岛素耐量试验。收集下丘脑、肝脏和脂肪组织以及血清用于蛋白质和mRNA分析。与对照组相比，所有HFD组均较重，具有较高的脂肪-瘦体组成和较慢的葡萄糖处置。与低LA组相比，两个高LA组的体重较小，但具有统计学显著性。下丘脑和周围组织中炎症和代谢标志物的分子数据仍在收集中。这些结果表明，在小鼠中，相对于低脂饮食，高度饱和和高度不饱和的HFD都诱导肥胖，但与目前认为的相反，饱和脂肪不会导致更大的体重增加，并且可能不太致肥胖
One of the significant changes in the Western diet, preceding and continuing concurrently with the obesity epidemic, has been a change in fatty acid profile from saturated and monounsaturated and towards polyunsaturated fatty acids, primarily n‐6 linoleic acid (LA). The objective of this study is to observe the obesity inducing ability of different types of fatty acids within a high fat diet (HFD). Forty‐eight male C57BL6/J mice were divided into 4 dietary treatment groups (n=12): control (10% kcals fat) and 3 HFDs (45% kcals fat) with the different percentages of LA constituting the 45% fat. The first HFD had 1% kcals from linoleic acid (LA); the second HFD had 15% LA; and the third had 22.5% kcals from LA. The primary source of LA was soybean oil and the primary source of saturated fat was coconut oil. During the 12‐week study, body weight and food intake were recorded. After 12 weeks, MRI body composition, indirect calorimetry and glucose and insulin tolerance tests were performed. Hypothalamic, liver and adipose tissues and blood serum were collected for protein and mRNA analysis. All HFD groups were heavier, had higher fat to lean body composition and slower glucose disposal compared to the control group. The two high LA groups had a small but statistically significant higher bodyweight than the low LA group. Molecular data on inflammatory and metabolic markers in the hypothalamus and peripheral tissues are still being collected. These results suggest that in the mouse both highly saturated and highly unsaturated HFDs induce obesity relative to low fat diet but that contrary to current thought saturated fats do not cause greater weight gain and are potentially less obesogenic