Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation.
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation.
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DOI：
10.1016/j.stem.2015.11.004
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发表时间：
2016-02-04
期刊：
影响因子：
23.9
通讯作者：
Lehmann R
中科院分区：
文献类型：
--
作者：
Sanchez CG;Teixeira FK;Czech B;Preall JB;Zamparini AL;Seifert JR;Malone CD;Hannon GJ;Lehmann R
复杂的调控网络调节干细胞的行为和对组织生长、修复和体内平衡的贡献。然而，对控制干细胞自我更新和分化的网络的充分理解尚未实现。为了系统地剖析这些网络并鉴定其组成部分，我们在雌性果蝇生殖系干细胞（GSC）中进行了无偏倚的、全转录组的体内RNAi筛选。基于表征的细胞缺陷，我们将646个鉴定的基因分为表型和功能组，并揭示了一套全面的调控GSC维持、存活和分化的网络。这项分析揭示了一个意想不到的作用，核糖体组装因子在控制干细胞胞质分裂。此外，我们的数据表明，从自我更新到分化的过渡依赖于增强的核糖体生物合成伴随着增加的蛋白质合成。总的来说，这些结果详细说明了控制干细胞稳态的广泛遗传网络，并突出了分化过程中蛋白质合成的复杂调控。
Complex regulatory networks regulate stem cell behavior and contributions to tissue growth, repair, and homeostasis. A full understanding of the networks controlling stem cell self-renewal and differentiation, however, has not yet been realized. To systematically dissect these networks and identify their components, we performed an unbiased, transcriptome-wide in vivo RNAi screen in female Drosophila germline stem cells (GSCs). Based on characterized cellular defects, we classified 646 identified genes into phenotypic and functional groups, and unveiled a comprehensive set of networks regulating GSC maintenance, survival, and differentiation. This analysis revealed an unexpected role for ribosomal assembly factors in controlling stem cell cytokinesis. Moreover, our data show that the transition from self-renewal to differentiation relies on enhanced ribosome biogenesis accompanied by increased protein synthesis. Collectively, these results detail the extensive genetic networks that control stem cell homeostasis and highlights intricate regulation of protein synthesis during differentiation.