Post-conditioning Preserves Glycolytic ATP During Early Reperfusion: A survival Mechanism for the Reperfused Heart
Post-conditioning Preserves Glycolytic ATP During Early Reperfusion: A survival Mechanism for the Reperfused Heart
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DOI：
10.1159/000185547
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Zazueta, Cecilia
中科院分区：
文献类型：
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作者：
Correa, Francisco;Garcia, Noemi;Zazueta, Cecilia
背景：从无氧代谢向有氧代谢过渡期间的糖酵解活动已被证明是离体心功能恢复的关键。这项工作的目的是调查糖酵解途径在保护条件后心脏的心功能方面的相关性。方法：通过测定GLUT-4插入、葡萄糖消耗和乳酸产生来评价心脏糖酵解途径的激活。在工作心脏中加入碘乙酸和2-脱氧-D-葡萄糖，以评价糖酵解抑制在后处理保护中的作用。结果：大鼠离体心在长时间缺血后再灌流前进行后处理，可促进心脏机械功能的恢复，GLUT-4转位持续增加，糖酵解途径在缺血再灌流早期被激活。碘乙酸酯抑制后处理的保护作用，但不影响线粒体的氧化能力。在灌流2-脱氧-D-葡萄糖的后处理心脏和仅在再灌流期间给予碘乙酸酯的心脏中，观察到糖酵解对维持再灌流早期机械功能的贡献。结论：在后处理心脏中，在再灌流早期，无氧能量代谢的功能区隔对心脏的再灌注损伤具有重要的保护作用。版权所有(C)2008 S.Karger AG，巴塞尔
Background: Glycolytic activity during the transition period from anaerobic to aerobic metabolism has been demonstrated to be critical for heart recovery in isolated reperfused hearts. The purpose of this work was to investigate the relevance of the glycolytic pathway in preserving the cardiac function of post-conditioned hearts. Methods: The activation of the glycolytic pathway in post-conditioned hearts was evaluated by measuring GLUT-4 insertion, glucose consumption and lactate production. Iodoacetic acid and 2-deoxy-D-glucose were administrated to the working hearts to evaluate the effect of glycolytic inhibition in the post-conditioning protective effect. Results: Post-conditioning maneuvers applied to isolated rat hearts, after prolonged ischemia and before reperfusion, promoted recovery of cardiac mechanical function with sustained increase of GLUT-4 translocation and activation of the glycolytic pathway during ischemia and early reperfusion. Iodoacetate inhibited the protective effect of post-conditioning, without affecting the mitochondrial oxidative capacity. Glycolysis contribution to maintain mechanical function at early reperfusion was observed in post-conditioned hearts perfused with 2-deoxy-D-glucose and in hearts in which iodoacetate was administered only during reperfusion. Conclusion: It is concluded that in the post-conditioned heart, a functional compartmentation of anaerobic energy metabolism, at early reperfusion, plays a significant role in cardiac protection against reperfusion damage. Copyright (C) 2008 S. Karger AG, Basel