Comparison of Normal and Pre-Eclamptic Placental Gene Expression: A Systematic Review with Meta-Analysis.
Comparison of Normal and Pre-Eclamptic Placental Gene Expression: A Systematic Review with Meta-Analysis.
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DOI：
10.1371/journal.pone.0161504
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Woodman A
中科院分区：
文献类型：
--
作者：
Brew O;Sullivan MH;Woodman A
子痫前期是一种严重的多因素妊娠期疾病。它与胎盘基因表达的变化有关。最近用微阵列分析胎盘基因的转录图谱提供了更好的机会来定义这种疾病的分子病理学。然而，胎盘基因表达在PE中的变化程度尚不完全清楚。我们对已发表的PE和正常妊娠(NP)对照胎盘RNA芯片进行了系统的综述，以描述NP和PE胎盘基因表达的相似性和差异性，并研究这些差异如何有助于疾病的分子病理学。共有167个微阵列样本可用于荟萃分析。我们发现一组基因在PE和NP中的表达模式是相同的。该综述还确定了一组基因(PE特有基因)，包括一个子集，仅在子痫前期胎盘中显著下调(p<；0.05)。利用类别预测分析，我们进一步确定了与PE高度相关的88个基因的表达(p<；0.001)，其中10个基因(LEP、hTRA4、SPAG4、LHb、TREM1、FSTL3、cgb、inha、pror和ltf)在p<；我们的综述还表明，目前正在研究的可能在PE胎盘中起重要作用的基因中，约有30%在PE胎盘中没有一致和显著的影响。我们建议进一步的工作来确认PE独特的和相关的基因的作用，目前还没有被研究在疾病的分子病理学中。
Pre-eclampsia (PE) is a serious multi-factorial disorder of human pregnancy. It is associated with changes in the expression of placental genes. Recent transcription profiling of placental genes with microarray analyses have offered better opportunities to define the molecular pathology of this disorder. However, the extent to which placental gene expression changes in PE is not fully understood. We conducted a systematic review of published PE and normal pregnancy (NP) control placental RNA microarrays to describe the similarities and differences between NP and PE placental gene expression, and examined how these differences could contribute to the molecular pathology of the disease. A total of 167 microarray samples were available for meta-analysis. We found the expression pattern of one group of genes was the same in PE and NP. The review also identified a set of genes (PE unique genes) including a subset, that were significantly (p < 0.05) down-regulated in pre-eclamptic placentae only. Using class prediction analysis, we further identified the expression of 88 genes that were highly associated with PE (p < 0.05), 10 of which (LEP, HTRA4, SPAG4, LHB, TREM1, FSTL3, CGB, INHA, PROCR, and LTF) were significant at p < 0.001. Our review also suggested that about 30% of genes currently being investigated as possibly of importance in PE placenta were not consistently and significantly affected in the PE placentae. We recommend further work to confirm the roles of the PE unique and associated genes, currently not being investigated in the molecular pathology of the disease.