Whole-genome analysis of vancomycin-resistant Enterococcus faecium causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavaria, Germany
Whole-genome analysis of vancomycin-resistant Enterococcus faecium causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavaria, Germany
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DOI：
10.1093/jac/dkaa041
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发表时间：
2020-06-01
影响因子：
5.2
通讯作者：
Valenza, Giuseppe
中科院分区：
文献类型：
--
作者：
Eisenberger, David;Tuschak, Christian;Valenza, Giuseppe
目的：由于治疗选择有限，由万古霉素耐药屎肠球菌（VREfm）引起的感染是一个主要的公共卫生问题。在侵袭性VREfm分离株中，ST 117、ST 80和ST 78代表了德国MLST最常检出的ST。在本研究中，我们通过WGS调查了从德国巴伐利亚州不同医院内爆发中回收的VREfm分离株的遗传多样性。方法：2018年1月至2019年4月期间，通过WGS调查了来自巴伐利亚州8家不同医院的99株非复制VREfm分离株的遗传多样性。详细地说，复杂类型（CT）的核心基因组MLST鉴定。结果：本研究的大多数分离株（76%）属于3个主要克隆群，它们至少出现在3家医院：ST 80/CT 1065 vanB（n = 45; 6家医院）、ST 117/CT 71 vanB（n = 11; 4家医院）和ST 78/CT 894 like vanA（n = 19; 3家医院）。此外，分离的优势谱系ST 80/CT 1065 vanB显示了36 SNPs的最大差异，显示由SNP analysis.Conclusions：全基因组分析VREfm引起医院内爆发表明，在巴伐利亚医院设置的地方性克隆谱系，即ST 80/CT 1065 vanB，ST 117/CT 71 vanB和ST 78/CT 894喜欢vanA的发生。要更好地了解影响上述谱系成功传播的因素，还需要进一步的研究。
Objectives: Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) represent a major public health concern due to limited treatment options. Among invasive isolates of VREfm, ST117, ST80 and ST78 represent the most frequently detected STs by MLST in Germany. In this study, we investigated the genetic diversity of isolates of VREfm recovered from different nosocomial outbreaks in Bavaria, Germany, byWGS.Methods: Between January 2018 and April 2019, 99 non-replicate isolates of VREfm originating from nosocomial outbreaks at eight different hospitals in Bavaria were investigated for genetic diversity by WGS. In detail, complex types (CTs) were identified by core-genome MLST. Furthermore, an SNP analysis was performed for all VREfm strains.Results: Most of the isolates of this study (76%) belonged to three major clonal groups, which occurred in at least three hospitals: ST80/CT1065 vanB (n = 45; six hospitals), ST117/CT71 vanB (n = 11; four hospitals) and ST78/CT894like vanA (n = 19; three hospitals). Moreover, isolates of the predominant lineage ST80/CT1065 vanB showed a maximumdifference of 36 SNPs as revealed by SNP analysis.Conclusions: Whole-genome analysis of VREfm causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavarian hospital settings, namely ST80/CT1065 vanB, ST117/CT71 vanB and ST78/ CT894like vanA. Further studies are needed for a better understanding of the factors affecting the successful spread of the above-mentioned lineages.