Preparation of laser micropore porcine acellular dermal matrix for skin graft: An experimental study
Preparation of laser micropore porcine acellular dermal matrix for skin graft: An experimental study
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DOI：
10.1016/j.burns.2006.08.039
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发表时间：
2007-09-01
期刊：
影响因子：
2.7
通讯作者：
Sheng Zhi-Yong
中科院分区：
文献类型：
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作者：
Chai Jia-Ke;Liang Li-Ming;Sheng Zhi-Yong
在我们之前的研究中，我们在临床实践中使用了由网状猪脱细胞真皮基质（PADM）和薄的刃厚自体表皮组成的复合移植物来覆盖全层烧伤创面。但由于烧伤创面真皮基质分布不均匀，可能会产生一定程度的收缩。本研究在激光辅助下制备了含PADM的复合皮片，以提高烧伤创面的愈合质量。方法：采用胰蛋白酶/TritonX-100法制备PADM。用激光打孔器在PADM上产生微孔。微孔间距为0.8、1.0、1.2 ~ 1.5mm。在144只SD大鼠背部造成全层缺损创面。将大鼠随机分为6组：Ⅰ ~ Ⅳ组创面分别植入4种不同距离的带微孔PADM和自体刃厚皮片;网状组创面植入网状PADM和自体刃厚皮片;对照组创面植入单纯自体刃厚皮片。术后定期组织学观察创面愈合情况。计算创面愈合率和收缩率。结果如下：植入后6周，植入物I组和II组的伤口愈合率与对照组无统计学差异，但显著高于补片组。植入后4周和6周，植入物III组和IV组的伤口愈合率低于补片组和对照组。术后4周、6周时，Ⅰ、Ⅱ组创面收缩率明显低于对照组，术后6周时明显低于补片组。组织学检查显示上皮化良好，胶原纤维排列规则，基底膜结构完整。结论：激光预处理PADM（0.8或1.0mm）联合自体刃厚皮片移植可促进创面愈合。激光微孔间距为1.0mm的PADM是较好的选择。
In our previous study, we used composite grafts consisting of meshed porcine acellular dermal matrix (PADM) and thin split-thickness autologous epidermis to cover full thickness burn wounds in clinical practice. However, a certain degree of contraction might occur because the distribution of dermal matrix was not uniform in burn wound. in this study, we prepare a composite skin graft consisting of PADM with the aid of laser to improve the quality of healing of burn wound. Methods: PADM was prepared by the trypsin/Triton X-100 method. Micropores were produced on the PADM with a laser punch. The distance between micropores varied from 0.8, 1.0, 1.2 to 1.5 mm. Full thickness defect wounds were created on the back of 144 SD rats. The rats were randomly divided into six groups: micropore groups I-IV in which the wound were grafted with PADM with micropores, in four different distances, respectively and split-thickness autograft; mesh group rats received meshed PADM graft and split-thickness autograft; control group received simple split-thickness autografting. The status of wound healing was histologically observed at regular time points after surgery. The wound healing rate and contraction rate were calculated. Results: The wound healing rate in micropore groups I and II was not statistically different from that in control group, but was significantly higher than that in mesh group 6 weeks after grafting. The wound healing rate in micropore groups III and IV was lower than that in mesh and control groups 4 and 6 weeks after grafting. The wound contraction rate in micropore groups I and II was remarkably lower than that in control group 4 and 6 weeks after surgery and it was significantly much lower than that in mesh group 6 weeks after surgery. Histological examination revealed good epithelization, regularly arranged collagenous fibers and integral structure of basement membrane. Conclusion: Laser micropore PADM (0.8 or 1.0 mm in distance) grafting in combination with split-thickness autografting can improve wound healing. The PADM with laser micropores in 1.0 mm distance is the better choice.