Lipid mobilization and locomotor stimulation in Gryllus bimaculatus by topically applied adipokinetic hormone
Lipid mobilization and locomotor stimulation in Gryllus bimaculatus by topically applied adipokinetic hormone
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DOI：
10.1111/j.1365-3032.2004.00378.x
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发表时间：
2004-06
影响因子：
1.5
通讯作者：
M. Lorenz;R. Zemek;D. Kodrík;R. Socha
中科院分区：
文献类型：
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作者：
M. Lorenz;R. Zemek;D. Kodrík;R. Socha
抽象的。测量了 3 日龄成年雌性 Gryllus bimaculatus (de Geer)（Ensifera，Gryllidae）24 小时内的步行活动。发现暗期的运动活动水平很高，比光期高两到三倍。开灯2 h后血淋巴中脂质滴度较低，关灯2 h后明显升高，但下一光相开灯2 h后，脂质滴度已降至基础水平。局部应用同源 Grybi 脂肪运动激素 (AKH)（20% 2-丙醇中 100 pmol）导致血淋巴脂质显着增加，与注射 AKH（水中 3 pmol）引起的最大增加相当。局部应用 AKH 还可以刺激蟋蟀的运动活动（100 pmol Grybi-AKH 的最大刺激量是 1.8 倍）。结果表明 AKH 快速渗透角质层。据推测，AKH 至少部分通过增加血淋巴脂质滴度来刺激运动活动；然而，不能排除 AKH 通过直接神经调节作用刺激运动活动。
Abstract. Walking activity of 3‐day‐old adult female Gryllus bimaculatus (de Geer) (Ensifera, Gryllidae) was measured over 24 h. A high level of locomotor activity during the scotophase was found, which was two‐ to three‐fold higher than that during the photophase. The titre of lipid in the haemolymph was relatively low 2 h after lights on, increased significantly 2 h after lights off, although, 2 h after lights on in the next photophase, the lipid titre had decreased to the basal level. Topical application of homologous Grybi‐adipokinetic hormone (AKH) (100 pmol in 20% 2‐propanol) led to a significant increase in haemolymph lipids, comparable with the maximal increase caused by injection of AKH (3 pmol in water). Topical application of AKH also stimulated locomotor activity in crickets (maximal stimulation 1.8‐fold with 100 pmol Grybi‐AKH). The results suggest that AKH penetrates the cuticle quickly. It is assumed that AKH stimulates locomotory activity at least in part via the increase of haemolymph lipid titres; however, the stimulation of locomotor activity via a direct neuromodulatory effect of AKH cannot be excluded.