QUARTZ-CRYSTAL MICROBALANCE FOR THE DETECTION OF MICROGRAM QUANTITIES OF HUMAN SERUM-ALBUMIN - RELATIONSHIP BETWEEN THE FREQUENCY CHANGE AND THE MASS OF PROTEIN ADSORBED
QUARTZ-CRYSTAL MICROBALANCE FOR THE DETECTION OF MICROGRAM QUANTITIES OF HUMAN SERUM-ALBUMIN - RELATIONSHIP BETWEEN THE FREQUENCY CHANGE AND THE MASS OF PROTEIN ADSORBED
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DOI：
10.1021/ac00068a036
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发表时间：
1993-10-15
影响因子：
7.4
通讯作者：
IKEDA, H
中科院分区：
文献类型：
--
作者：
MURATSUGU, M;OHTA, F;IKEDA, H
研制了一种用于微量白蛋白检测的压电免疫传感器。人血清白蛋白（HSA）在0.1-100 μ g mL-1的范围内可以使用流动池检测;免疫传感器是足够灵敏的监测白蛋白尿的水平。该免疫传感器对牛血清白蛋白没有反应，仅对HSA有反应，这意味着对HSA的特异性高。 研究了压电活性石英晶体（DELTAM）的频率变化（DELTAF）与单位面积吸附量之间的关系。用放射性同位素标记的抗HSA或HSA估计DELTAM。当抗-HSA吸附到晶体表面上或HSA结合到由晶体支撑的抗-HSA上时，\DELTAF/DELTAM\的值大于从理论预测的值（Sauerbrey方程）。此外，HSA的Δ F/Δ TAM大于抗HSA。
We have developed a piezoelectric immunosensor for the detection of microalbumin. Human serum albumin (HSA) in the range 0.1-100 mug mL-1 could be detected using a flow cell; the immunosensor is sensitive enough to monitor levels of albuminuria. The immunosensor did not respond to bovine serum albumin, only to HSA, implying that the specificity for HSA was high. We investigated the relationship between the frequency change (DELTAF) and adsorption per unit area of piezoelectrically active quartz crystal (DELTAM). DELTAM was estimated with radioisotope-labeled anti-HSA or HSA. When anti-HSA was adsorbed onto the surface of the crystal or HSA was bound to anti-HSA supported by the crystal, values of \DELTAF/DELTAM\ were larger than the value predicted from theory (Sauerbrey's equation). Furthermore, \DELTAF/DELTAM\ for HSA was larger than that for anti-HSA.