Multiple markers pyrosequencing reveals highly diverse and host-specific fungal communities on the mangrove trees Avicennia marina and Rhizophora stylosa
Multiple markers pyrosequencing reveals highly diverse and host-specific fungal communities on the mangrove trees Avicennia marina and Rhizophora stylosa
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DOI：
10.1111/j.1574-6941.2011.01236.x
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发表时间：
2012-02-01
影响因子：
4.2
通讯作者：
Record, Eric
中科院分区：
文献类型：
--
作者：
Arfi, Yonathan;Buee, Marc;Record, Eric
真菌是红树林生态过程和营养网的重要参与者。虽然红树林潮间带的腐殖真菌已被广泛研究，但对该生态系统中真菌群落的多样性和结构以及病原菌和互利共生菌等功能群的重要性知之甚少。使用标签编码的454焦磷酸测序的ITS 1，ITS 2，nu-ssu-V5和nu-ssu-V7区域，我们研究和比较的海洋和空气中的部分白骨壤marina和红海榄树在新喀里多尼亚的红树林的真菌群落。共分析了209544个读数，对应于数千个分子操作分类单位（OTU）。物种分布有明显的地带性，大部分OTU都是在所研究的微生境中发现的。子囊菌是优势门（82%），担子菌是非常罕见的（3%），和15%的序列对应于未知的类群。我们的研究结果表明，主机的特异性是一个关键因素，在高度多样化的真菌群落的分布，在空中和潮间带部分的树木。本研究还验证了标签编码焦磷酸测序中多个标记的有用性，以巩固和完善分类多样性的评估。
Fungi are important actors in ecological processes and trophic webs in mangroves. Although saprophytic fungi occurring in the intertidal part of mangrove have been well studied, little is known about the diversity and structure of the fungal communities in this ecosystem or about the importance of functional groups like pathogens and mutualists. Using tag-encoded 454 pyrosequencing of the ITS1, ITS2, nu-ssu-V5 and nu-ssu-V7 regions, we studied and compared the fungal communities found on the marine and aerial parts of Avicennia marina and Rhizophora stylosa trees in a mangrove in New Caledonia. A total of 209 544 reads were analysed, corresponding to several thousand molecular operational taxonomic units (OTU). There is a marked zonation in the species distribution, with most of the OTU being found specifically in one of the microhabitat studied. Ascomycetes are the dominant phylum (82%), Basidiomycetes are very rare (3%), and 15% of the sequences correspond to unknown taxa. Our results indicate that host specificity is a key factor in the distribution of the highly diverse fungal communities, in both the aerial and intertidal parts of the trees. This study also validates the usefulness of multiple markers in tag-encoded pyrosequencing to consolidate and refine the assessment of the taxonomic diversity.