Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch
Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch
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DOI：
10.1023/a:1025985625706
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发表时间：
2004
影响因子：
2.6
通讯作者：
M. Yi;Y. Li;J. Liu;L. Zhou;Q. Yu;J. Gui
中科院分区：
文献类型：
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作者：
M. Yi;Y. Li;J. Liu;L. Zhou;Q. Yu;J. Gui
在雌核发生中，来自相关物种的精子激活卵子和胚胎发育，但通常不会对后代产生遗传贡献。然而，在银鲫中，雌核发育后代常常表现出一些显然源自异源精子供体的表型。这种异源发生的父本效应在用钝鼻黑鳊（Megaloabrama amblycephalaYin）精子授精后对克隆 E 卵进行冷处理产生的人工克隆 F 中表现得很突出。核型分析显示，除了从母体克隆E遗传的156条正常染色体外，克隆F的不同个体中还存在5-15条多余的微染色体。从显微解剖的微染色体中制备了绘画探针，并用于研究这些微染色体的起源。克隆F的每条微染色体和钝鼻黑鲷的4对染色体上均检测到强阳性信号，而克隆E的染色体上未检测到信号。这一结果表明，钝鼻黑鲷的一些父本染色体片段已被整合到人工克隆F中。因此，异源发生的操纵可能为鱼类育种提供不同物种之间DNA转移的独特方法。
In gynogenesis, sperm from related species activates egg and embryonic development, but normally does not contribute genetically to the offspring. In gibel carp,Carassius auratus gibelioBloch, however, gynogenetic offspring often show some phenotypes apparently derived from the heterologous sperm donor. This paternal effect of allogynogenesis is outstanding in an artificial clone F produced by cold treatment of clone E eggs after insemination with blunt-nose black bream (Megaloabrama amblycephalaYin) sperm. Karyotype analysis revealed 5–15 supernumerary microchromosomes in different individuals of clone F in addition to 156 normal chromosomes inherited from the maternal clone E. A painting probe was prepared from the microdissected microchromosomes, and used to investigate the origin of these microchromosomes. Strong positive signals were detected on each microchromosomes of clone F and on 4 pairs of chromosomes in blunt-nose black bream, whereas no signals were detected on the chromosomes of clone E. This result indicates that some paternal chromosome fragments of blunt-nose black bream have been incorporated into the artificial clone F. Therefore, the manipulation of allogynogenesis may provide a unique method to transfer DNA between diverse species for fish breeding.