Light Emission as a Probe of Charge Separation and Recombination in the Photosynthetic Apparatus: Relation of Prompt Fluorescence to Delayed Light Emission and Thermoluminescence
Light Emission as a Probe of Charge Separation and Recombination in the Photosynthetic Apparatus: Relation of Prompt Fluorescence to Delayed Light Emission and Thermoluminescence
复制标题
光发射作为光合装置中电荷分离和重组的探针：瞬发荧光与延迟光发射和热释光的关系
DOI：
10.1007/978-1-4020-3218-9_13
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
I. Vass
中科院分区：
文献类型：
--
作者：
E. Tyystjärvi;I. Vass
光合作用机制发出一小部分吸收的光作为一个高度信息化的废物过程。发射的光可以在照明期间作为即时荧光或在照明关闭后作为延迟发光来观察。发光以延迟发光（DLE）的形式作为时间的函数或以热释光（TL）的形式作为温度的函数来测量。荧光和发光主要来源于光系统II。叶绿素荧光产率受光系统II的电子受体QA的还原状态的调制，而发光发射的瞬时强度反映了光合电子转移链II的电子受体和电子供体之间的电荷重组反应的速率。荧光法和发光法在光合作用光反应的研究中已被证明是非常有价值的工具。在这一章中，我们回顾了DLE，TL和光合系统荧光的基础知识和最新进展，特别集中在这些过程的动力学和三种测量类型之间的联系上
The photosynthetic machinery emits a fraction of absorbed light as a highly informative waste process. The emitted light can be observed as prompt fluorescence during illumination or as delayed luminescence after illumination is turned off. Luminescence is measured in the form of delayed light emission (DLE) as a function of time or in the form of thermoluminescence (TL) as a function of temperature. Both fluorescence and luminescence originate mainly in Photosystem II. The yield of chlorophyllafluorescence is modulated by the reduction state of the electron acceptor QAof Photosystem II, whereas the momentary intensity of luminescence emission reflects the rates of charge recombination reactions between electron acceptors and electron donors of the photosynthetic electron transfer chain II. Both fluorescence and luminescence methods have proved to be invaluable tools in the studies of photosynthetic light reactions. In this chapter, we review the basics and the recent advances in DLE, TL and fluorescence of photosynthetic systems, concentrating particularly on the kinetics of these processes and on the links between the three types of measurement