Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples
Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples
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DOI：
10.1016/j.foodchem.2009.05.015
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发表时间：
2010-01-15
期刊：
影响因子：
8.8
通讯作者：
Zhang, Jun-Hui
中科院分区：
文献类型：
--
作者：
Zhou, Yu;Li, Yan-Song;Zhang, Jun-Hui
Brevetoxin B (PbTx-2) was covalently linked to carrier protein bovine serum albumin and human gamma globulin. A monoclonal antibody against PbTx-2, which showed high cross-reactivity values with PbTx-1, PbTx-3 and PbTx-9 (more than 89%) was obtained from ascites and some characteristics of monoclonal antibody were studied. An direct competitive enzyme-linked immunosorbent assay (ELISA) for detection of PbTxs was developed, which showed an IC50 value of 5.3 ng mL(-1) with a detection limit of 0.6 ng well(-1). The recoveries of PbTxs from cockle (88.4%-102.3%) and oyster (89.4%-104.3%) demonstrated that the matrices of cockle and oyster where PbTxs are found do not interfere with the assay. The newly developed competitive ELISA appears to be a reliable and useful method for mass monitoring of PbTxs in mollusk. (C) 2009 Elsevier Ltd. All rights reserved.
