Using xCELLigence RTCA Instrument to Measure Cell Adhesion
Using xCELLigence RTCA Instrument to Measure Cell Adhesion
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DOI：
10.21769/bioprotoc.2646
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发表时间：
2017-12-20
期刊：
影响因子：
0.8
通讯作者：
Ivaska, Johanna
中科院分区：
文献类型：
--
作者：
Hamidi, Hellyeh;Lilja, Johanna;Ivaska, Johanna
细胞与邻近细胞和底层细胞外基质（ECM）的粘附是多细胞生物存在的基本要求。因此，细胞粘附的形成、稳定性和解离受到空间和时间的严格控制，复杂的粘附机制内的扰动与多种人类病理相关。在这里，我们概述了一个简单的方案，用于使用 xCELLigence 实时细胞分析 (RTCA) 系统监测细胞粘附到 ECM 的变化，例如，在基因操作或感兴趣的蛋白质过度表达后或对药物治疗的反应。
Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are associated with a variety of human pathologies. Here, we outline a simple protocol to monitor alterations in cell adhesion to the ECM, for example, following genetic manipulations or overexpression of a protein of interest or in response to drug treatment, using the xCELLigence real-time cell analysis (RTCA) system.