New evidence for geographic variation in the role of human papillomavirus in tonsillar carcinogenesis
New evidence for geographic variation in the role of human papillomavirus in tonsillar carcinogenesis
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DOI：
10.1080/00313020701230823
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发表时间：
2007-04-01
期刊：
影响因子：
4.5
通讯作者：
Rose, Barbara R.
中科院分区：
文献类型：
--
作者：
Li, Wei;Tran, Nham;Rose, Barbara R.
目标：我们以前对澳大利亚新南威尔士州和中国东北吉林省扁桃体癌的研究提供了证据，证明这些癌症中归因于人乳头瘤病毒（HPV）的比例在地理上存在差异。方法：采用PCR/测序法检测49例香港扁桃体癌组织中HPV DNA，免疫组化半定量检测p16（INK 4A）、pRb蛋白、cyclin D1和p53蛋白表达，并与新南威尔士州和吉林省扁桃体癌组织进行比较。结果如下：在31名香港癌症患者中，有9名（29%）经聚合酶链反应检测为人类乳头瘤病毒阳性，而新南威尔士州的阳性率为46%，吉林省的阳性率为0%。HPV阳性与女性、年轻、p16（INK 4A）过表达、pRb和cyclin D1缺失有关。HPV阳性和HPV阴性癌症患者的5年疾病特异性生存率分别为82%和42%。HPV感染状态与细胞蛋白表达的关系与新南威尔士州和吉林省一致。HPV相关癌症的比例反映了口咽癌的相对发病率在这些regions.Conclusions：HPV是负责在香港患者的扁桃体癌的一小部分。在香港、新南威尔士州和吉林省，HPV引起的肿瘤比例的差异可能是由于不同人群的环境、文化或遗传因素所致。
Aims: Our previous studies of tonsillar cancers from New South Wales, Australia, and Jilin Province in the north-east of China, provided evidence that the proportion of these cancers attributable to human papillomavirus (HPV) varies geographically. This study provides the first data on HPV in tonsillar cancers from Hong Kong.Methods: A total of 49 Hong Kong tonsillar cancers were analysed for HPV DNA by PCR/sequencing and for p16(INK4A) retinoblastoma (pRb) protein, cyclin D1 and p53 expression by semiquantitative immunohistochemistry as evidence of virus causality.Results were compared with those from New South Wales and Jilin Province. Results: Of the 31 Hong Kong cancers with amplifiable DNA, nine (29%) were HPV positive by PCR compared with 46% from New South Wales and 0% from Jilin Province. HPV positivity correlated with female gender, young age, overexpression of p16(INK4A) and loss of pRb and cyclin D1. Five-year disease-specific survival for patients with HPV positive and HPV negative cancers was 82 and 42%, respectively. Relationships between HPV status and cell protein expression in Hong Kong cancers were consistent with those from New South Wales and Jilin Province. The proportion of HPV-associated cancers reflected the relative incidence of oropharyngeal cancer in these regions.Conclusions: HPV is responsible for a small proportion of tonsillar cancers in Hong Kong patients. Differences in the proportions of tumours attributable to HPV in Hong Kong, New South Wales and Jilin Province may be due to environmental, cultural or genetic factors in the different populations.