Water column anammox and denitrification in a temperate permanently stratified lake (Lake Rassnitzer, Germany)
Water column anammox and denitrification in a temperate permanently stratified lake (Lake Rassnitzer, Germany)
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DOI：
10.1016/j.syapm.2009.07.009
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发表时间：
2009-12-01
影响因子：
3.4
通讯作者：
Kuypers, Marcel M. M.
中科院分区：
文献类型：
--
作者：
Hamersley, M. Robert;Woebken, Dagmar;Kuypers, Marcel M. M.
在一个年度周期内，我们研究了德国一个恢复的矿坑湖缺氧水柱中厌氧氨氧化和反硝化作用产生微生物N-2的情况。我们使用连续泵送取样器获得了高分辨率的水化学剖面。Rassnitzer湖在大约29米深的地方永久分层，将缺氧水带到盐水浓度梯度以下。混合层硝酸盐平均浓度约为200mmol.L-1，但在缺氧底层水中浓度降至零。而氨氮在混合层中为-5mU·m-1·L-1，而在缺氧水中增加到沉积物附近的约600mU·m-1·L-1。在1月和10月，N-15示踪测量检测到Anammox活性(在(NH_4+)-N-15-修正培养中最大值504nmolN(2)L(-1)d(-1))，但没有反硝化作用。相反，在5月份，N-2的产生以反硝化为主(最大值为74nmolN-2 L-1d(-1))。5月份的Anammox活性明显低于10月份，表现为Anammox的速率(最大值为6nmolN，与(NO3-)-N-15孵育的L-1d(-1))，以及Anammox细菌的相对和绝对细胞丰度(分别占细菌总数的0.56%和2.7x10(4)vs.5.2×10(4)anammox细胞mL(-1))(通过催化报告沉积-荧光原位杂交(CARD-FISH)和Anammox细菌特异性探针(CARD-FISH)定量)。利用厌氧氨氧化细菌特异性16S rRNA基因克隆文库对厌氧氨氧化细菌多样性进行了研究。大多数厌氧氨氧化细菌序列与广泛存在的丝状念珠菌/雀形目相关。然而，我们也发现了与假丝酵母S.wagneri和假丝酵母Brocadia fulgida相关的序列，这表明该湖具有与河口沉积物相当的高度厌氧氨氧化细菌多样性。(C)2009年爱思唯尔股份有限公司。版权所有。
We studied microbial N-2 production via anammox and denitrification in the anoxic water column of a restored mining pit lake in Germany over an annual cycle. We obtained high-resolution hydrochemical profiles using a continuous pumping sampler. Lake Rassnitzer is permanently stratified at ca. 29 m depth, entraining anoxic water below a saline density gradient. Mixed-layer nitrate concentrations averaged ca. 200 mu mol L-1, but decreased to zero in the anoxic bottom waters. In contrast, ammonium was < 5 mu mol L-1 in the mixed layer but increased in the anoxic waters to ca. 600 mu mol L-1 near the sediments. In January and October, N-15 tracer measurements detected anammox activity (maximum 504 nmol N(2)L(-1)d(-1) in (NH4+)-N-15-amended incubations), but no denitrification. In contrast, in May, N-2 production was dominated by denitrification (maximum 74 nmol N-2 L-1 d(-1)). Anammox activity In May was significantly lower than in October, as characterized by anammox rates (maximum 6 vs. 16 nmol N, L-1 d(-1) in incubations with (NO3-)-N-15), as well as relative and absolute anammox bacterial cell abundances (0.56% vs. 0.98% of all bacteria, and 2.7 x 10(4) vs. 5.2 x 10(4) anammox cells mL(-1), respectively) (quantified by catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) with anammox bacteria-specific probes). Anammox bacterial diversity was investigated with anammox bacteria-specific 16S rRNA gene clone libraries. The majority of anammox bacterial sequences were related to the widespread Candidatus Scalindua sorokinii/brodae cluster. However, we also found sequences related to Candidatus S. wagneri and Candidatus Brocadia fulgida, which suggests a high anammox bacterial diversity in this lake comparable with estuarine sediments. (C) 2009 Elsevier GmbH. All rights reserved.