Analysis of Tissue-specific RNA Editing Events of Genes Involved in RNA Editing in Arabidopsis thaliana
Analysis of Tissue-specific RNA Editing Events of Genes Involved in RNA Editing in Arabidopsis thaliana
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DOI：
10.1007/s12374-018-0452-5
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发表时间：
2019-10-01
影响因子：
2.9
通讯作者：
Tsukahara, Toshifumi
中科院分区：
文献类型：
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作者：
Qulsum, Umme;Azad, Md. Thoufic Anam;Tsukahara, Toshifumi
在植物中，RNA编辑是转录本的转录后修饰，通常发生在叶绿体和线粒体中。在开花植物中，不仅PPR，而且非PPR蛋白，如Morf/RIP，Orrm和OZ都参与了多样化的RNA编辑复合体。在拟南芥中，在核、叶绿体和线粒体转录本中预测了12种类型的RNA编辑模式。在这项研究中，在参与RNA编辑的基因家族中检测到了组织特异性的RNA编辑事件。收集了不同发育阶段的拟南芥不同发育阶段的组织，包括4、8和12天的幼苗，以及16、21、27和32天的叶、茎、柄和根。在靶基因中发现了9种RNA编辑事件，包括C-to-U、U-to-C、A-to-I、A-to-C、A-to-U、G-to-A、G-to-C、U-to-A和U-to-G。大部分编辑事件发生在幼苗和叶片中，少数发生在茎组织中。在12d龄幼苗和21d龄叶片中分别检测到广泛的U-C编辑(60%)和A-G编辑(54%)。这是在植物中发现的第一个组织特异性核RNA编辑事件的实验报告。本研究将为揭示植物的RNA编辑机制提供重要信息。
In plants, RNA editing is a post-transcriptional modification of transcripts and commonly occurs in plastids and mitochondria. In the case of flowering plants, not only PPR but also non-PPR proteins, such as MORF/RIP, ORRM and OZ partake in the diverse RNA editing complex. In Arabidopsis thaliana, 12 types of RNA editing patterns have been predicted in nuclear, chloroplast, and mitochondrial transcripts. In this study, tissue-specific RNA editing events were detected in gene families involved in RNA editing. Different Arabidopsis tissues at variable developmental stages, including 4-, 8-, and 12-d-old seedlings and 16-, 21-, 27-, and 32-d-old leaves, stems, stipes and roots, were collected and used for this study. Nine types of RNA editing events, including C-to-U, U-to-C, A-to-I, A-to-C, A-to-U, G-to-A, G-to-C, U-to-A and U-to-G, were identified in target genes. Most of the editing events occurred in seedlings and leaves and a few in stem tissues. Extensive U-to-C editing (60%) and A-to-G editing (54%) was detected in 12-d-old seedlings and 21-d-old leaves, respectively. This is the first experimental report of tissue-specific nuclear RNA editing events found in plants. This study will provide important information for revealing the mechanism of RNA editing in plants.