Protein synthesis inhibitor cycloheximide dose-dependently decreases formalin-induced c-Fos protein and behavioral hyperalgesia in rats
Protein synthesis inhibitor cycloheximide dose-dependently decreases formalin-induced c-Fos protein and behavioral hyperalgesia in rats
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DOI：
10.1016/s0304-3940(97)00321-2
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发表时间：
1997-05-16
影响因子：
2.5
通讯作者：
Sun, WZ
中科院分区：
文献类型：
--
作者：
Hou, WY;Shyu, BC;Sun, WZ
我们以前已经证明c-fos反义寡核苷酸剂量依赖性地抑制福尔马林诱导的c-Fos蛋白和行为痛觉过敏。为了检测蛋白质的重新合成是否是外周炎症后持续性疼痛的发展所必需的，我们观察了福尔马林诱导的脊髓c-Fos蛋白和蛋白质合成抑制剂放线菌酮预处理后的伤害性行为。环己酰亚胺剂量依赖性地抑制福尔马林诱导的脊髓c-Fos蛋白和强直性伤害性反应。这些结果提供了进一步的支持的假设，从头蛋白质的合成是必不可少的行为痛觉过敏的发展。(C)1997 Elsevier Science爱尔兰有限公司
We had previously demonstrated that c-fos antisense oligodeoxynucleotides dose-dependently suppressed formalin-induced c-Fos protein and behavioral hyperalgesia, To test whether de novo protein synthesis is required for the development of persistent pain after peripheral inflammation, we observed formalin-induced spinal c-Fos protein and nociceptive behaviors following pretreatment with cycloheximide, a protein synthesis inhibitor. Cycloheximide dose-dependently inhibited formalin-induced spinal c-Fos protein and tonic nociceptive responses, The possible non-specific effects other than protein synthesis inhibition on nociceptive behavior were carefully discussed and excluded. These results provide further support to the hypothesis that de novo protein synthesis is essential for the development of behavioral hyperalgesia. (C) 1997 Elsevier Science Ireland Ltd.