Decline after immobilisation and recovery after remobilisation of synovial fluid IL1, TIMP, and chondroitin sulphate levels in young beagle dogs
Decline after immobilisation and recovery after remobilisation of synovial fluid IL1, TIMP, and chondroitin sulphate levels in young beagle dogs
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DOI：
10.1136/ard.60.1.55
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发表时间：
2001-01-01
影响因子：
27.4
通讯作者：
Kiviranta, I
中科院分区：
文献类型：
--
作者：
Haapala, J;Arokoski, JPA;Kiviranta, I
目的：监测幼年比格犬膝关节(窒息)关节滑液中软骨和滑膜代谢标志物的浓度。方法：17只犬右后肢屈曲制动11周。同时，对侧左膝暴露于增加的负重。调整期持续了50周。以同窝小鼠为对照。免疫分析法检测关节灌洗液中白介素1α(IL1α)的浓度，提取法测定磷脂酶A(PLA)的活性，Alcian蓝沉淀法测定硫酸软骨素(CS)浓度，用生物素标记的HA结合络合物、基质金属蛋白酶3(MMP3)和金属蛋白酶组织抑制因子1(TIMP 1)用类ELISA法测定透明质酸(HA)浓度，用双抗体夹心ELISA法测定关节灌洗液中IL 1α的浓度，光镜下观察滑膜炎症程度。不动能降低关节灌洗液中IL-1α的水平(p
Objective-To monitor the concentration of markers of cartilage and synovium metabolism in the knee (stifle) joint synovial fluid of young beagles subjected to immobilisation and subsequent remobilisation.Methods-The right hind limb of 17 dogs was immobilised in flexion for 11 weeks. Simultaneously, the contralateral left knee was exposed to increased weight bearing. The remobilisation period lasted 50 weeks. Litter mates served as controls. The concentration in joint lavage fluid of interleukin 1 alpha (IL1 alpha) was measured by immunoassay, the activity of phospholipase A, (PLA,) was determined by an extraction method, chondroitin sulphate (CS) concentration by precipitation with Alcian blue, hyaluronan (HA) by an ELISA-like assay using biotinylated HA-binding complexes,matrix metalloproteinase 3 (MMP-3), and tissue inhibitor of metalloproteinases 1 (TIMP-1) by sandwich ELISA, and synovitis was scored by light microscopy.Results-Synovitis or effusion was absent in all experimental and control groups. Immobilisation decreased the joint lavage fluid levels of IL1 alpha (p