Quantum dot nanobead-based fluorescent immunochromatographic assay for simultaneous quantitative detection of fumonisin B1, dexyonivalenol, and zearalenone in grains
Quantum dot nanobead-based fluorescent immunochromatographic assay for simultaneous quantitative detection of fumonisin B1, dexyonivalenol, and zearalenone in grains
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基于量子点纳米珠的荧光免疫色谱法同时定量检测谷物中的伏马菌素 B-1、右旋镰刀菌烯醇和玉米赤霉烯酮
DOI：
10.1016/j.foodcont.2020.107331
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发表时间：
2020-11-01
期刊：
影响因子：
6
通讯作者：
Yan, Yaxian
中科院分区：
文献类型：
--
作者：
Hou, Silu;Ma, Jingjiao;Yan, Yaxian
谷物中的霉菌毒素严重威胁着人类和动物的健康。在这项研究中，我们展示了一种快速和灵敏的荧光免疫层析检测伏马菌素B-1（FB 1），脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）的方法，使用量子点纳米珠（QDNBs，100 nm）的发展。纳米球包覆对量子点荧光信号的放大可以显著提高免疫层析试纸条的灵敏度。在竞争性免疫层析平台上，将荧光强度为615 nm的QDNBs分别与抗FB 1、DON和ZEN单克隆抗体偶联，制备了检测探针（FB 1-McAb@QDNBs、DON-McAb@QDNBs和ZEN-McAb@QDNBs）。QDNB表面修饰有羧基官能团，这有利于抗体的偶联，并显著提高测试条的灵敏度。与传统的ELISA相比，该单抗具有更高的灵敏度，IC 50分别为12.66 ng/mL（FB 1），2.97 ng/mL（DON）和0.87 ng/mL（ZEN），线性范围分别为2.295 ~ 69.867 ng/mL（FB 1），0.465 ~ 16.19 ng/mL（DON），和0.295 ng/mL至2.575 ng/mL（ZEN）。通过检测加标样品和天然样品，评价了已建立的试纸的准确度、重复性和专属性。此外，在同时定量检测三种真菌毒素方面，所开发的测试条和LC-MS/MS之间观察到高度一致性。该方法可用于农产品中真菌毒素的同时定量检测。
The mycotoxins in grains is a serious threat to the health of humans and animals. In this study, we demonstrated an approach for development of a fast and sensitive fluorescent immunochromatographic assay for fumonisin B-1 (FB1), deoxynivalenol (DON) and zearalenone (ZEN) detection using quantum dots nanobeads (QDNBs, 100 nm). Amplification of the fluorescence signal of the quantum dot by nanosphere coating can significantly improve the sensitivity of immunochromatographic test strip. The detection probes (FB1-McAb@QDNBs, DON-McAb@QDNBs, and ZEN-McAb@QDNBs) were prepared using the QDNBs with 615 nm emissions coupling with three monoclonal antibodies (McAb) against FB1, DON, and ZEN, respectively, in a competitive immunochromatography platform. The surface of the QDNBs is modified with a carboxyl functional group, which facilitates the coupling of the antibody and significantly improves the sensitivity of test strip. Compared with traditional ELISA, the immunoassay based on the McAb@QDNBs is more sensitive, with IC50 of 12.66 ng/mL (FB1), 2.97 ng/mL (DON), and 0.87 ng/mL (ZEN), of which the linear range were from 2.295 ng/mL to 69.867 ng/mL (FB1), 0.465 ng/mL to 16.19 ng/mL (DON), and 0.295 ng/mL to 2.575 ng/mL (ZEN), respectively. The accuracy, repeatability, and specificity of the established test strip was evaluated by testing the spiked and natural samples. Furthermore, a high agreement was observed between the developed test strips and LC-MS/MS in simultaneously and quantitative detection of three mycotoxins. In brief, the developed approach was feasible and could be employed for simultaneously and quantitative mycotoxins detection in agricultural products.