Evaluation of a quantitative determination of CD4 and CD8 molecules as an alternative to CD4+ and CD8+ T lymphocyte counts in Africans
Evaluation of a quantitative determination of CD4 and CD8 molecules as an alternative to CD4+ and CD8+ T lymphocyte counts in Africans
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DOI：
10.1046/j.1365-3156.1999.00362.x
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发表时间：
1999-02-01
影响因子：
3.3
通讯作者：
Ledru, E
中科院分区：
文献类型：
--
作者：
Diagbouga, S;Durand, G;Ledru, E
在发达国家，HIV感染者的监测通常通过CD4+T淋巴细胞绝对计数来确定。流式细胞术的参考程序价格昂贵，需要复杂的仪器和经过专门培训的操作员。由于这些限制，发展中国家通常无法进行 CD4 计数。 Capcellia 测定是一种酶联免疫测定法，用于定量测定 CD4 和 CD8 分子。我们在西非对从 39 名未感染艾滋病毒和 44 名感染艾滋病毒的成年受试者采集的血液样本评估了这种方法。 CD4浓度范围根据疾病的临床阶段确定。然后我们在艾滋病毒感染者身上研究了两种方法之间的关系。 Spearman 等级相关性为 0.61（95% 置信区间：0.38-0.76，P < 0.0001）。然而，一致性限度的确定揭示了两种方法之间的差异，特别是对于 CD4 计数 > 0.4 X 10(9)/l，对此进行了讨论。我们得出的结论是，Capcellia 测定是一种在流式细胞仪无法使用的情况下确定免疫抑制水平的便捷方法，并且可以与 CD4 T 淋巴细胞计数相补充。
In the developed word,monitoring HIV-infected patients is routinely determined by CD4 + T lymphocyte absolute counts. The reference procedure, flow cytometry, is expensive, requires sophisticated instrumentation and operators with specific training. Due to these limitations, CD4 counting is often unavailable in developing countries. The Capcellia assay is an enzyme-linked immunoassay for quantitative determination of CD4 and CD8 molecules. We evaluated this method in West Africa on blood samples collected from 39 HIV-uninfected and 44 HIV-infected adult subjects. CD4 concentration ranges were determined according to the clinical stages of the disease. We then studied the relationship between the two methods in the HIV-infected patients. The Spearman's rank correlation was 0.61 (95 % confidence interval: 0.38-0.76, P < 0.0001). Nevertheless, determination of limits of agreement revealed discrepancies between the two methods, especially for CD4 counts > 0.4 X 10(9)/l, which are discussed. We conclude that the Capcellia assay is a convenient means to determine the immunodepression level where flow cytometric instrumentation is unavailable, and can be complementary to CD4 T lymphocyte enumeration.