Glycobiology Aspects of the Periodontal Pathogen Tannerella forsythia.
Glycobiology Aspects of the Periodontal Pathogen Tannerella forsythia.
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DOI：
10.3390/biom2040467
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发表时间：
2012-10-12
期刊：
影响因子：
5.5
通讯作者：
Schäffer C
中科院分区：
文献类型：
--
作者：
Posch G;Sekot G;Friedrich V;Megson ZA;Koerdt A;Messner P;Schäffer C
糖生物学对于牙周病原体Tannerella asthia是重要的，影响细菌的细胞完整性，其生活方式和毒力潜力。该细菌具有独特的革兰氏阴性细胞包膜，其具有糖基化的表面（S-）层作为最外层装饰，其被提议通过粗糙的脂多糖锚定。S层聚糖具有结构4-MeO-b-ManpNAcCONH 2-（1→3）-[Pse 5Am 7 Gc-（2→4）-]-b-ManpNAcA-（1→4）-[4-MeO-a-Galp-（1→2）-]-a-Fucp-（1→4）-[-a-Xylp-（1→3）-]-b-GlcpA-（1 → 3）-[-b-Digp-（1→2）-]-a-Galp，并与D（S/T）（A/I/L/M/T/V）氨基酸基序内的不同丝氨酸和苏氨酸残基连接。此外，其他几种坦纳氏菌蛋白质也被S层寡糖修饰，表明存在一般的O-糖基化系统。蛋白质O-糖基化影响T.因为在确定的突变体中存在的截短的S-层聚糖有利于生物膜形成，因此，虽然S层也被证明是一种毒力因子，并延迟宿主先天免疫系统对细菌的识别，但糖基化对调节宿主免疫力的贡献目前正在解开。最近的研究表明，Tannerella表面糖基化在抑制Th 17介导的中性粒细胞浸润牙龈组织中具有作用。T.该菌表达一种强大的酶谱，包括几种糖苷酶，如唾液酸酶，其与特定的生长需求有关，并参与触发宿主组织破坏。本文综述了近年来T.艾西娅
Glycobiology is important for the periodontal pathogen Tannerella forsythia, affecting the bacterium’s cellular integrity, its life-style, and virulence potential. The bacterium possesses a unique Gram-negative cell envelope with a glycosylated surface (S-) layer as outermost decoration that is proposed to be anchored via a rough lipopolysaccharide. The S-layer glycan has the structure 4‑MeO-b-ManpNAcCONH2-(1→3)-[Pse5Am7Gc-(2→4)-]-b-ManpNAcA-(1→4)-[4-MeO-a-Galp-(1→2)-]-a-Fucp-(1→4)-[-a-Xylp-(1→3)-]-b-GlcpA-(1→3)-[-b-Digp-(1→2)-]-a-Galp and is linked to distinct serine and threonine residues within the D(S/T)(A/I/L/M/T/V) amino acid motif. Also several other Tannerella proteins are modified with the S‑layer oligosaccharide, indicating the presence of a general O‑glycosylation system. Protein O‑glycosylation impacts the life-style of T. forsythia since truncated S-layer glycans present in a defined mutant favor biofilm formation. While the S‑layer has also been shown to be a virulence factor and to delay the bacterium's recognition by the innate immune system of the host, the contribution of glycosylation to modulating host immunity is currently unraveling. Recently, it was shown that Tannerella surface glycosylation has a role in restraining the Th17-mediated neutrophil infiltration in the gingival tissues. Related to its asaccharolytic physiology, T. forsythia expresses a robust enzymatic repertoire, including several glycosidases, such as sialidases, which are linked to specific growth requirements and are involved in triggering host tissue destruction. This review compiles the current knowledge on the glycobiology of T. forsythia.