NO+, NO(CENTER-DOT), AND NO- DONATION BY S-NITROSOTHIOLS - IMPLICATIONS FOR REGULATION OF PHYSIOLOGICAL FUNCTIONS BY S-NITROSYLATION AND ACCELERATION OF DISULFIDE FORMATION
NO+, NO(CENTER-DOT), AND NO- DONATION BY S-NITROSOTHIOLS - IMPLICATIONS FOR REGULATION OF PHYSIOLOGICAL FUNCTIONS BY S-NITROSYLATION AND ACCELERATION OF DISULFIDE FORMATION
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DOI：
10.1006/abbi.1995.1231
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发表时间：
1995-04-20
影响因子：
3.9
通讯作者：
STAMLER, JS
中科院分区：
文献类型：
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作者：
ARNELLE, DR;STAMLER, JS
S-亚硝基硫醇的生物效应归因于 S-N 键的均裂并释放一氧化氮 (NO.)。 NO. 费率通过监测 pH 7.4 下氧合肌红蛋白向高铁肌红蛋白的氧化来测定几种 S-亚硝基硫醇的释放；氧合肌红蛋白氧化的半衰期从数秒到数小时不等。S-亚硝基硫醇与含硫醇的氨基酸、肽和蛋白质之间的转亚硝基反应比自发的 NO 发生得更快，这表明亚硝基硫醇具有作为亚硝酰基 (NO+) 供体的能力。释放，S-亚硝基二硫醇的分解作为氮氧化物与蛋白质上邻位硫醇反应的模型进行了检查。二硫化物的快速形成伴随着羟胺和一氧化二氮的形成，表明硝酰基（NO-）的释放。总而言之，这些模型研究证明了 S-亚硝基硫醇在生理条件下充当 NO+、NO. 和 NO- 供体的能力，转亚硝化和加速二硫化物形成表明通过亚硝基硫醇中介调节蛋白质功能的机制，并支持这样的观点：S-亚硝基硫醇的生物活性可能与异解和均解机制有关。 分解。 (C) 1995 学术出版社
The biological effects of S-nitrosothiols have been attributed to homolytic cleavage of the S-N bond with release of nitric oxide (NO.). Rates of NO. release from several S-nitrosothiols were determined by monitoring the oxidation of oxymyoglobin to metmyoglobin at pH 7.4; half-lives for oxymyoglobin oxidation ranged from seconds to hours, Transnitrosation reactions between S-nitrosothiols and thiol-containing amino acids, peptides, and proteins, which indicate the ability of nitrosothiols to act as nitrosyl (NO+) donors, occurred more rapidly than spontaneous NO. release, Decomposition of S-nitrosodithiols were examined as models for the reaction of nitrogen oxides with vicinal thiols on proteins. Rapid disulfide formation was accompanied by formation of hydroxylamine and nitrous oxide, indicative of nitroxyl (NO-) release. Taken together, these model studies demonstrate the ability of S-nitrosothiols to act as NO+, NO., and NO- donors under physiological conditions, Transnitrosation and acceleration of disulfide formation suggest mechanisms of regulation of protein function through the intermediacy of nitrosothiols, and support the notion that biological activities of S-nitrosothiols may be associated with heterolytic as well as homolytic mechanisms of decomposition. (C) 1995 Academic Press, Inc.