Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications
Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications
复制标题
DOI：
10.1016/j.ymeth.2003.11.023
复制
发表时间：
2004-06-01
期刊：
影响因子：
4.8
通讯作者：
Ciccarone, VC
中科院分区：
文献类型：
--
作者：
Dalby, B;Cates, S;Ciccarone, VC
Lipofectamine 2000 是一种基于阳离子脂质体的试剂，可使用简单的实验方案在体外多种哺乳动物细胞类型中提供高转染效率和高水平的转基因表达。最佳转染效率和随后的细胞活力取决于许多实验变量，例如细胞密度、脂质体和 DNA 浓度、脂质体-DNA 复合时间以及培养基成分（例如抗生素和血清）的存在或不存在。这些因素在 Lipofectamine 2000 介导的转染中的重要性将与一些具体应用一起讨论：原代神经元的转染、高通量转染和小干扰 RNA 的递送。 (C) 2003 Elsevier Inc. 保留所有权利。
Lipofectamine 2000 is a cationic liposome based reagent that provides high transfection efficiency and high levels of transgene expression in a range of mammalian cell types in vitro using a simple protocol. Optimum transfection efficiency and subsequent cell viability depend on a number of experimental variables such as cell density, liposome and DNA concentrations, liposome-DNA complexing time, and the presence or absence of media components such as antibiotics and serum. The importance of these factors in Lipofectamine 2000 mediated transfection will be discussed together with some specific applications: transfection of primary neurons, high throughput transfection, and delivery of small interfering RNAs. (C) 2003 Elsevier Inc. All rights reserved.