Adiponectin as inducer of inflammatory and apoptosis involving in immune defense in lamprey.
Adiponectin as inducer of inflammatory and apoptosis involving in immune defense in lamprey.
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DOI：
10.1016/j.fsi.2019.04.045
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发表时间：
2019-07
影响因子：
4.7
通讯作者：
Kejia Zhang;Zhao Tai;Qing Han;Y. Pang;Qingwei Li
中科院分区：
文献类型：
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作者：
Kejia Zhang;Zhao Tai;Qing Han;Y. Pang;Qingwei Li
脂联素（APN）是脂肪细胞分泌的一种重要的细胞因子，负责调节多种生物学功能。然而，在七鳃鳗APN基因和它的确切功能仍然不明。本研究克隆了L-APN的全长cDNA序列，其编码的蛋白由267个氨基酸组成，具有球状结构域。免疫组化和流式细胞仪检测结果显示APN蛋白分布于多种组织。L-APN在神经上体（SB）和白细胞中的表达在革兰氏阴性菌、革兰氏阳性菌和poly（I：C）的作用下差异性上调。炎症细胞因子的表达水平上调，并刺激SB细胞与重组APN的促凋亡作用。此外，L-APN还能抑制细胞增殖，使细胞生长阻滞于G1期。综上所述，七鳃鳗APN蛋白在抑制细胞增殖、诱导炎性细胞因子产生和促进细胞凋亡等方面发挥重要作用，并参与免疫应答和免疫防御。我们的数据为APN基因结构和功能的进化起源提供了新的见解。
Adiponectin (APN) is an important cytokine secreted by fat cells that is responsible for regulating numerous biological functions. However, the APN gene in lamprey and its precise function remain unidentified. In this study, the full-length cDNA sequence of L-APN was cloned, and it encoded a protein of 267 amino acid residues with a globular domain. The results of immunohistochemistry and FACS assays showed that APN protein was distributed in multiple tissues. L-APN expression in the supraneural body (SB) and leukocytes was differentially upregulated in response to Gram-negative bacteria, Gram-positive bacteria and poly (I:C). The expression levels of inflammatory cytokines were upregulated, and a proapoptotic effect was stimulated in SB cells treated with recombinant APN. Furthermore, L-APN could inhibit cell proliferation and arrest cell growth in the G1 phase. In summary, the APN protein from the lamprey plays an important role in inhibiting cell proliferation, inducing the production of inflammatory cytokines and promoting cell apoptosis, and it is also involved in immune responses and immune defenses. Our data provide insights into the evolutionary origin of the structure and function of APN gene.