A replication-competent chimera of plant and animal viruses.
A replication-competent chimera of plant and animal viruses.
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具有复制能力的植物和动物病毒嵌合体。
DOI：
10.1006/viro.2001.1306
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Dolja,ValerianV
中科院分区：
文献类型：
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作者：
Peng,Chih-Wen;Peremyslov,ValeraV;Snijder,EricJ;Dolja,ValerianV
人类、动物、真菌和植物病毒编码木瓜蛋白酶样蛋白酶，其在多蛋白加工、RNA合成和病毒-宿主相互作用中起作用。为了比较不同木瓜蛋白酶样蛋白酶的功能概况，我们用来自马动脉炎病毒（EAV）、口蹄疫病毒（FMDV）和真菌病毒CHV 1的蛋白酶基因替换了甜菜黄化病毒（BYV）的蛋白酶基因。我们发现，虽然每一种外源蛋白酶都能有效地处理病毒多蛋白，但只有EAV蛋白酶支持嵌合BYV在植物原生质体中的有力复制。这一结果表明，BYV和EAV的蛋白酶，而不是FMDV或CHV 1，提供了一个功能，这是基因组复制的关键，是从多蛋白加工分离。对BYV-EAV嵌合体的进一步表征表明，BYV蛋白酶也是病毒侵入和细胞间移动所必需的。因此，相同的病毒蛋白可以联合收割机结合植物和动物病毒共有的复制相关功能和病毒-宿主相互作用中的专门功能。
Human, animal, fungal, and plant viruses encode papain-like proteinases that function in polyprotein processing, RNA synthesis, and virus–host interactions. To compare the functional profiles of diverse papain-like proteinases, we replaced a proteinase gene of the beet yellows virus (BYV) with those derived from equine arteritis virus (EAV), foot-and-mouth disease virus (FMDV), and the fungal virus CHV1. We found that, although each of the foreign proteinases efficiently processed the viral polyprotein, only the EAV proteinase supported vigorous replication of the chimeric BYV in plant protoplasts. This result demonstrated that the proteinases of BYV and EAV, but not FMDV or CHV1, provide a function that is critical for genome replication and that is separable from polyprotein processing. Further characterization of the BYV–EAV chimera revealed that BYV proteinase is also required for virus invasion and cell-to-cell movement. Thus, the same viral protein can combine both replication-related functions shared by plant and animal viruses and specialized functions in virus–host interactions.