Identification of candidate genes for grain number in rice (Oryza sativa L.)
Identification of candidate genes for grain number in rice (Oryza sativa L.)
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DOI：
10.1007/s10142-010-0167-2
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发表时间：
2010-08-01
影响因子：
2.9
通讯作者：
Singh, Nagendra
中科院分区：
文献类型：
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作者：
Deshmukh, Rupesh;Singh, Abhinay;Singh, Nagendra
每穗实粒数多是水稻重要的产量构成性状。利用QTL定位和转录组分析相结合的方法，筛选出控制籽粒数的候选基因。利用166个平均分布在水稻12条染色体上的SSR标记构建了一个框架连锁图谱。利用3年的QTL表型数据，对由高粒数Pusa 1266与低粒数Pusa Basmati 1杂交产生的一组重组自交系进行QTL定位，在第4染色体长臂上检测到1个QTLqGN4-1，对粒数有主效作用。该QTL与每穗一次枝、二次枝和每株穗数的主效QTL共定位。通过使用6个额外的标记对该区域进行定向富集化，将QTL区间缩小到11.1 cM(0.78 Mbp)。基因芯片转录组分析显示，在穗部发育早期，qGN4-1区域有8个基因在双亲之间存在差异表达。在小麦、大麦、玉米、高粱和蕨类中检测了该QTL和潜在候选QTL的同源性，以进一步验证这种关联。
Large number of well-filled grains per panicle is an important yield component trait in rice. A combination of QTL mapping and transcriptome profiling was used to identify candidate genes for grain number. A framework linkage map was constructed using 166 SSR markers evenly distributed over the 12 rice chromosomes. QTL mapping using 3 years phenotyping data on a set of recombinant inbred lines derived from a cross between Pusa 1266 (high grain number) and Pusa Basmati 1 (low grain number) identified one consistent QTL qGN4-1 on the long arm of chromosome 4 with major effect on grain number. This QTL was co-localized with major QTLs for primary and secondary branches per panicle, and number of panicles per plant. The QTL interval was narrowed down to 11.1 cM (0.78 Mbp) by targeted enrichment of the region with six additional markers. Microarray transcriptome profiling revealed eight genes in the qGN4-1 region differentially expressed between the two parents during early panicle development. Synteny of this QTL and potential candidates was examined in wheat, barley, maize, sorghum, and Brachypodium to further validate the association.