Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins.
Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins.
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DOI：
10.1007/978-1-0716-1205-7_26
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Padera TP
中科院分区：
文献类型：
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作者：
Pereira ER;Kedrin D;Padera TP
淋巴结微环境是极其动态的，并且通过重编程免疫细胞、基质细胞和内皮细胞来响应宿主中的免疫刺激。在正常的生理条件下，淋巴结将启动适当的免疫反应，以清除宿主可能遇到的外部威胁。然而，在转移性疾病中，癌细胞通常定植于局部淋巴结，破坏免疫功能，甚至离开淋巴结以产生额外的转移。了解癌细胞如何进入，定植，存活，增殖以及与淋巴结中的其他细胞类型相互作用是具有挑战性的。在这里，我们描述了使用光转换荧光蛋白标记和跟踪癌细胞的命运，一旦他们进入淋巴结。
The lymph node microenvironment is extremely dynamic and responds to immune stimuli in the host by reprogramming immune, stromal, and endothelial cells. In normal physiological conditions, the lymph node will initiate an appropriate immune response to clear external threats that the host may experience. However, in metastatic disease, cancer cells often colonize local lymph nodes, disrupt immune function, and even leave the lymph node to create additional metastases. Understanding how cancer cells enter, colonize, survive, proliferate, and interact with other cell types in the lymph node is challenging. Here, we describe the use of photoconvertible fluorescent proteins to label and trace the fate of cancer cells once they enter the lymph node.