Transport ATPase: thimerosal inhibits the Na+ +K+-dependent ATPase activity without diminishing the Na+-dependent ATPase activity.
Transport ATPase: thimerosal inhibits the Na+ +K+-dependent ATPase activity without diminishing the Na+-dependent ATPase activity.
复制标题
转运 ATP 酶：硫柳汞抑制 Na K 依赖性 ATP 酶活性，但不减弱 Na 依赖性 ATP 酶活性。
DOI：
10.1016/0006-291x(76)90549-0
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发表时间：
1976
影响因子：
3.1
通讯作者：
A. Askari
中科院分区：
文献类型：
--
作者：
G. Henderson;A. Askari
A guinea pig kidney membrane preparation was incubated with thimerosal and then thoroughly washed. Comparison of the properties of the native and the modified membranes showed that (a) Na++K+-dependent activity is substantially inhibited by thimerosal; (b) thimerosal does not diminish Na+-dependent ATPase activity; and (c) the thimerosal treated enzyme, like the native enzyme, is phosphorylated in the presence of Na+and ATP, and dephosphorylated upon the addition of K+. It is suggested that thimerosal does not affect the binding of ATP to the high-affinity catalytic site, but that it blocks the binding of ATP to a low affinity modifying site the occupation of which is essential for the dissociation of the stable K+-dephosphoenzyme and the recycling of the enzyme.
