LOCALIZATION IN DIPHTHERIA-TOXIN FRAGMENT-B OF A REGION THAT INDUCES PORE FORMATION IN PLANAR LIPID BILAYERS AT LOW PH
LOCALIZATION IN DIPHTHERIA-TOXIN FRAGMENT-B OF A REGION THAT INDUCES PORE FORMATION IN PLANAR LIPID BILAYERS AT LOW PH
复制标题
DOI：
10.1016/0014-5793(83)80941-7
复制
发表时间：
1983-01-01
期刊：
影响因子：
3.5
通讯作者：
RUYSSCHAERT, JM
中科院分区：
文献类型：
--
作者：
DELEERS, M;BEUGNIER, N;RUYSSCHAERT, JM
比如白喉毒素和N-末端片段B（Mr 23 000）区，CB 1片段B中部的溴化氰肽（Mr 13 000）在低pH条件下能诱导脂双层膜孔的形成（Mr 23 000和13 000）共有一个共同的片段（Mr 6300），该片段含有片段B的预测的两亲性、α-螺旋、横向脂质缔合结构域（Mr 2750）[J. Cell Biol.（1980）87，837-840]。因此，我们假设该结构域负责白喉毒素的孔形成能力[Proc. Natl. Acad. Sci. USA（1981）78，172-176]。孔形成的pH依赖性和存在的C-末端区域的CB 1的脯氨酸簇之间的关系提出。
Like diphtheria toxin and the N-terminal (Mr23 000) region of fragment B, CB1 (Mr13 000), the cyanogen bromide peptide located in the middle region of fragment B is able to induce pore formation in lipid bilayer membrane at low pH. These two peptides (Mr23 000 and 13 000) share a common segment (Mr6300) containing the predicted amphipathic, α-helical, transverse lipid-associating domain (Mr2750) of fragment B[J. Cell Biol. (1980) 87, 837–840]. Therefore, we postulated this domain to be responsible for the pore formation ability of diphtheria toxin [Proc. Natl. Acad. Sci. USA (1981) 78, 172–176]. A relationship between the pH dependency of pore formation and the presence of a cluster of prolines in the C-terminal region of CB1 is proposed.