Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices.
Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices.
复制标题
相干抗斯托克斯拉曼光谱（CARS）在脑切片髓鞘形成成像中的应用。
DOI：
10.3791/64013
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发表时间：
2022-07-22
影响因子：
1.2
通讯作者：
Klug, Achim
中科院分区：
文献类型：
--
作者：
McCullagh, Elizabeth A.;Poleg, Shani;Stich, Dominik;Moldovan, Radu;Klug, Achim
相干反斯托克斯拉曼光谱（汽车）是化学家和物理学家用来产生分子特征振动的相干信号的经典技术。然而，这些振动特征也是解剖组织（如大脑）内分子的特征，使其越来越适用于神经科学应用。例如，汽车可以通过特异性激发这些分子内的化学键来测量脂质，从而允许量化组织的不同方面，例如参与神经传递的髓鞘。此外，与通常用于定量髓磷脂的其他技术相比，汽车还可以设置为与免疫荧光技术兼容，允许与其他标记物如钠通道或突触传递的其他组分共标记。髓鞘形成变化作为脱髓鞘疾病如多发性硬化症或其他神经系统疾病如脆性X综合征或自闭症谱系障碍中固有的重要机制是一个新兴的研究领域。总之，汽车可以以创新的方式用于回答神经科学中的紧迫问题，并为与许多不同神经系统疾病相关的潜在机制提供证据。可视化髓鞘形成是许多研究神经系统的研究人员的重要目标。汽车是一种与免疫荧光兼容的技术，其可以对组织（例如大脑）内的脂质进行天然成像，从而照亮专门的结构（例如髓磷脂）。
Coherent anti-Stokes Raman spectroscopy (CARS) is a technique classically employed by chemists and physicists to produce a coherent signal of signature vibrations of molecules. However, these vibrational signatures are also characteristic of molecules within anatomical tissue such as the brain, making it increasingly useful and applicable for Neuroscience applications. For example, CARS can measure lipids by specifically exciting chemical bonds within these molecules, allowing for quantification of different aspects of tissue, such as myelin involved in neurotransmission. In addition, compared to other techniques typically used to quantify myelin, CARS can also be set up to be compatible with immunofluorescent techniques, allowing for co-labeling with other markers such as sodium channels or other components of synaptic transmission. Myelination changes as an inherently important mechanism in demyelinating diseases such as multiple sclerosis or other neurological conditions such as Fragile X Syndrome or autism spectrum disorders is an emerging area of research. In conclusion, CARS can be utilized in innovative ways to answer pressing questions in Neuroscience and provide evidence for underlying mechanisms related to many different neurological conditions. Visualizing myelination is an important goal for many researchers studying the nervous system. CARS is a technique that is compatible with immunofluorescence that can natively image lipids within tissue such as the brain illuminating specialized structures such as myelin.
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影响因子：
25
作者：
Barak, Boaz;Zhang, Zicong;Feng, Guoping
通讯作者：
Feng, Guoping
DOI：
10.3791/3564
发表时间：
2012-07-30
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gage, Gregory J;Kipke, Daryl R;Shain, William
通讯作者：
Shain, William
影响因子：
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作者：
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通讯作者：
Hampson, David R.
影响因子：
4.3
作者：
Lucas A;Poleg S;Klug A;McCullagh EA
通讯作者：
McCullagh EA
影响因子：
6.2
作者：
Bu, J;Banki, A;Nishiyama, A
通讯作者：
Nishiyama, A