LAR-PTPase cDNA transfection suppression of tumor growth of neu oncogene-transformed human breast carcinoma cells.
LAR-PTPase cDNA transfection suppression of tumor growth of neu oncogene-transformed human breast carcinoma cells.
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LAR-PTPase cDNA 转染抑制新癌基因转化的人乳腺癌细胞的肿瘤生长。
DOI：
10.1002/mc.2940140206
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发表时间：
1995
影响因子：
4.6
通讯作者：
Esselman,WJ
中科院分区：
文献类型：
--
作者：
Zhai,Y;Wirth,J;Kang,S;Welsch,CW;Esselman,WJ
The incidence of amplification ofneuoncogene‐encoded protein tyrosine kinase in human breast cancer strongly supports the concept that protein tyrosine phosphorylation and dephosphorylation are key regulatory mechanisms in the proliferation, differentiation, and neoplastic transformation of breast epithelial cells. We examined the potential regulatory role of protein tyrosine phosphatases (a) in the maintenance of cellular tyrosine phosphorylation by the introduction of leukocyte common‐antigen‐related PTPase (LAR‐PTPase) cDNA into a tumorigenic human breast carcinoma cell line that overexpressed p185neuprotein tyrosine kinase. The transfected human breast carcinoma cells expressed elevated levels of LAR‐PTPase as assessed by reverse transcription‐polymerase chain reaction and by analysis of LAR‐PTPase protein. The LAR‐PTPase‐transfected human breast carcinoma cells had a significantly (P<0.01) slower proliferation rate in vitro than control‐trans‐fected cells. When LAR‐PTPase‐transfected cells were inoculated into athymic nude mice, a consistent and significant (P<0.05) suppression of tumor growth was observed. These results provide evidence that a specific PTPase, LAR‐PTPase, can play a suppressive regulatory role in the tumor growth of human breast carcinoma cells that overexpress p185neuprotein tyrosine kinase. ©1995 Wiley‐Liss, Inc.
