EMPERIPOLESIS OF MARROW-CELLS WITHIN MEGAKARYOCYTES IN THE BONE-MARROW OF SUBLETHALLY IRRADIATED MICE
EMPERIPOLESIS OF MARROW-CELLS WITHIN MEGAKARYOCYTES IN THE BONE-MARROW OF SUBLETHALLY IRRADIATED MICE
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DOI：
10.1007/s002770050038
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发表时间：
1995-02-01
影响因子：
3.5
通讯作者：
DABROWSKI, Z
中科院分区：
文献类型：
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作者：
BOBIK, R;DABROWSKI, Z
对正常小鼠和X射线照射小鼠骨髓中巨核细胞癫痫的发生率进行了研究。两组小鼠接受单次5Gy全身照射，其中一组在照射前给予放射防护药物乙硫磷(WR-2721)治疗。来自第三组的小鼠保持不暴露于辐射，并作为对照。Wright-Giemsa染色的骨髓涂片在30天内每5天分析一次，从照射后1天开始。出现这种现象的巨核细胞的数量被确定，并表示为每个试验组的平均值。对照涂片中巨核细胞的发生率低于对照组涂片的15%，而单纯照射的小鼠巨核细胞的发生率增加到34%，照射前用WR-2721处理的小鼠的巨核细胞发生率增加到43%。在最后一种情况下，即放疗和放射防护药物治疗，注意到大核巨核细胞与红斑升高呈正相关。光镜下未见吞噬迹象；被吞噬的细胞保持不变，正常结构完好无损。粒细胞、红系细胞和淋巴样细胞似乎是最常被成熟的巨核细胞吞噬的骨髓细胞。单个巨核细胞的结合细胞数从1个到3个不等，但在巨核细胞中偶尔可见6个以上。根据我们的发现和对相关文献的回顾，我们认为红斑是一种有趣的细胞现象，与骨髓细胞快速通过骨髓-血液屏障，特别是通过巨核细胞的细胞质，以响应增加的细胞输送需求有关。照射后对细胞输送的高需求可能会导致某些成熟的骨髓细胞走跨巨核细胞的途径进入血液循环。照射似乎对红斑有立竿见影的效果(24小时后观察到)，提示体液因素参与了发病机制。
The incidence of megakaryocytic emperipolesis was studied in the bone marrow of normal and X-irradiated mice. Two groups of mice received total body irradiation with a single dose of 5 Gy and one of the two groups had been treated with a radioprotective drug, ethiofos (WR-2721), before irradiation. Mice from a third group remained unexposed to irradiation and served as controls. The Wright-Giemsa stained bone marrow smears were analyzed every 5 days during a 30-day period, starting 1 day after irradiation. The number of megakaryocytes exhibiting the phenomenon was determined and expressed as an average value for every experimental group. The frequency of megakaryocytic emperipolesis was less than 15% of megakaryocytes from control smears but increased to 34% in mice that had only been irradiated and to 43% when mice were treated with WR-2721 before irradiation. In the last case, i.e., irradiation and treatment with a radioprotective drug, a positive correlation between the macrocytic megakaryocytes and elevated emperipolesis was noted. Under light microscopy, there were no signs of phagocytosis; engulfed cells remained unaltered with their normal structure intact. Granulocytic, erythroid, and lymphoid cells appeared to be the most frequent marrow cells engulfed by mature megakaryocytes. The number of incorporated cells in one megakaryocyte ranged from 1 to 3, though occasionally more than 6 were seen in macrocytic megakaryocytes. Based on our findings and on a review of the associated literature, we believe emperipolesis is an interesting cellular phenomenon related to the fast passage of marrow cells across the marrow-blood barrier, especially through the cytoplasm of megakaryocytes in response to an increased demand for cell delivery. The high demand for cell delivery which occurs after irradiation may cause certain mature bone marrow cells to take a transmegakaryocyte path to enter the circulation of the blood. Irradiation seems to have an immediate effect (observed after 24 h) on emperipolesis, suggesting that a humoral factor is involved in the pathogenesis.